Regulation of Camphor Metabolism: Induction and Repression of Relevant Monooxygenases in Pseudomonas putida NCIMB 10007

Abstract

For the first time, the differential rates of synthesis of all the key monooxygenases involved in the catabolism by Pseudomonas putida NCIMB 10007 of bicyclic (rac)-camphor to ∆2,5-3,4,4-trimethylpimelyl-CoA, the first aliphatic pathway intermediate, have been determined to help establish the relevant induction profile of each of the oxygen-dependent enzymes. The efficacy of both relevant substrates and pathway metabolites as inducers has been established. Further, inhibitors with characterised functionality have been used to indicate that the pertinent regulatory controls operate at the level of transcription of the corresponding genes.

Keywords

• cytochrome P450 monooxygenase
• diketocamphane monooxygenase: 5-exo-hydroxycamphor dehydrogenase
• 2-oxo-∆3-4,5,5-trimethylcyclopentenylacetic acid monooxygenase
• Pseudomonas putida NCIMB 10007
• (rac)-camphor
• transcription
• rifampicin
• actinomycin D