The isolation of (6S, 9S)-cyclo (prolylvalyl) from marine actinomycete, by use of high speed contercurrent chromatography

Abstract

The marine actinomycete B 1758 came from the actinomycete collection of the Alfred Wegener Institute for Polar and Marine Research in Bremerhafen Germany. 1.079 g of raw extract was obtained by fermentation. Seven fractions were separated by column chromatography on silica gel. Fraction 4 was separated by high speed countercurrent chromatography. Fraction 4.6 yielded 5.2 mg of (6S, 9S)-cyclo(prolylvalyl).

Keywords

• (6s, 9s)-cyclo(prolylvalyl)
• marine actinomycete b 1758
• hsccc.