Lipidomics Analysis of Free Fatty Acids in Human Plasma of Healthy and Diabetic Subjects by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS)
Maroula G. Kokotou,
Christiana Mantzourani,
Charikleia S. Batsika,
Olga G. Mountanea,
Ioanna Eleftheriadou,
Ourania Kosta,
Nikolaos Tentolouris,
George Kokotos
Affiliations
Maroula G. Kokotou
Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece
Christiana Mantzourani
Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece
Charikleia S. Batsika
Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece
Olga G. Mountanea
Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece
Ioanna Eleftheriadou
Center of Excellence for Drug Design and Discovery, National and Kapodistrian University of Athens, 15771 Athens, Greece
Ourania Kosta
Diabetes Center, First Department of Propaedeutic and Internal Medicine, Medical School, National and Kapodistrian University of Athens, Laiko General Hospital, 15772 Athens, Greece
Nikolaos Tentolouris
Center of Excellence for Drug Design and Discovery, National and Kapodistrian University of Athens, 15771 Athens, Greece
George Kokotos
Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece
Targeted analytical methods for the determination of free fatty acids (FFAs) in human plasma are of high interest because they may help in identifying biomarkers for diseases and in monitoring the progress of a disease. The determination of FFAs is of particular importance in the case of metabolic disorders because FFAs have been associated with diabetes. We present a liquid chromatography-high resolution mass spectrometry (LC-HRMS) method, which allows the simultaneous determination of 74 FFAs in human plasma. The method is fast (10-min run) and straightforward, avoiding any derivatization step and tedious sample preparation. A total of 35 standard saturated and unsaturated FFAs, as well as 39 oxygenated (either hydroxy or oxo) saturated FFAs, were simultaneously detected and quantified in plasma samples from 29 subjects with type 2 diabetes mellitus (T2D), 14 with type 1 diabetes mellitus (T1D), and 28 healthy subjects. Alterations in the levels of medium-chain FFAs (C6:0 to C10:0) were observed between the control group and T2D and T1D patients.
