Frontiers in Veterinary Science (Jun 2022)
Complex Feline Disease Mapping Using a Dense Genotyping Array
- Isabel Hernandez,
- Jessica J. Hayward,
- Jeff A. Brockman,
- Michelle E. White,
- Michelle E. White,
- Lara Mouttham,
- Elizabeth A. Wilcox,
- Susan Garrison,
- Marta G. Castelhano,
- John P. Loftus,
- Filipe Espinheira Gomes,
- Cheryl Balkman,
- Marjory B. Brooks,
- Nadine Fiani,
- Marnin Forman,
- Tom Kern,
- Bruce Kornreich,
- Eric C. Ledbetter,
- Santiago Peralta,
- Angela M. Struble,
- Lisa Caligiuri,
- Elizabeth Corey,
- Lin Lin,
- Julie Jordan,
- Danny Sack,
- Adam R. Boyko,
- Leslie A. Lyons,
- Rory J. Todhunter
Affiliations
- Isabel Hernandez
- Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Jessica J. Hayward
- Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Jeff A. Brockman
- Pet Nutrition Center, Hill's Pet Nutrition, Topeka, KS, United States
- Michelle E. White
- Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, MA, United States
- Michelle E. White
- Vertebrate Genomics Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA, United States
- Lara Mouttham
- Cornell Veterinary Biobank, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Elizabeth A. Wilcox
- Cornell Veterinary Biobank, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Susan Garrison
- Cornell Veterinary Biobank, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Marta G. Castelhano
- Cornell Veterinary Biobank, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- John P. Loftus
- Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Filipe Espinheira Gomes
- Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Cheryl Balkman
- Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Marjory B. Brooks
- Department of Population Medicine and Diagnostic Services, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Nadine Fiani
- Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Marnin Forman
- Cornell University Veterinary Specialists, Stamford, CT, United States
- Tom Kern
- Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Bruce Kornreich
- Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Eric C. Ledbetter
- Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Santiago Peralta
- Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Angela M. Struble
- Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Lisa Caligiuri
- Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Elizabeth Corey
- Cornell Veterinary Biobank, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Lin Lin
- Cornell Veterinary Biobank, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Julie Jordan
- Cornell Veterinary Biobank, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Danny Sack
- Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Adam R. Boyko
- Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- Leslie A. Lyons
- Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO, United States
- Rory J. Todhunter
- Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States
- DOI
- https://doi.org/10.3389/fvets.2022.862414
- Journal volume & issue
-
Vol. 9
Abstract
The current feline genotyping array of 63 k single nucleotide polymorphisms has proven its utility for mapping within breeds, and its use has led to the identification of variants associated with Mendelian traits in purebred cats. However, compared to single gene disorders, association studies of complex diseases, especially with the inclusion of random bred cats with relatively low linkage disequilibrium, require a denser genotyping array and an increased sample size to provide statistically significant associations. Here, we undertook a multi-breed study of 1,122 cats, most of which were admitted and phenotyped for nine common complex feline diseases at the Cornell University Hospital for Animals. Using a proprietary 340 k single nucleotide polymorphism mapping array, we identified significant genome-wide associations with hyperthyroidism, diabetes mellitus, and eosinophilic keratoconjunctivitis. These results provide genomic locations for variant discovery and candidate gene screening for these important complex feline diseases, which are relevant not only to feline health, but also to the development of disease models for comparative studies.
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