Role of EBV infection in Type-1 Diabetic nephropathy pathogenesis with related to IL-12 level in patients

Abstract

Type 1 Diabetic nephropathy (T1DN) is the leading cause of chronic kidney disease globally The primary risk factors for the development of DN are chronic hyperglycemia and excessive blood pressure. In general, microalbuminuria screening should be done yearly, beginning 5 years after diagnosis in type 1 diabetes. This study design to investigate The role of IL-12 in the diagnostic of T1DN, and evaluation the role of IL-12 in patients infected and non-infected with EBVNA IgG. A case-control study design on 70 participates which included 30 patients with T1DN collected from nephrology department in Al-sadder medical city in AL- Najaf, from October 2022 till end of February 2023 and 40 control groups divided into two types, first 20 apparently healthy and 20 nephropathy patients. Blood sample was collected from all participants to detected IL-12 serum level and EBNA1 IgG by enzyme linked immunosorbent assay (ELISA). All patients were diagnosis by nephrology specialist. The results showed a that serum IL-12 levels were significantly higher in type 1 diabetic nephropathy patients (27.65 ± 3.78 pg/ml) than in the control groups (12.32± 3.41 pg/ml; 1.89 ±0.47 pg/ml) respectively at (P= 0.0001). Also The level of IL-12 was significantly elevated in T1DN patients infected with EBNA1 IgG (33.84 ± 4.47) in compare to patients non infected with EBNA1 IgG (13.21 ± 4.36) at P 0.01. Serum levels of IL-12 were significantly increased in T1DN Iraqi patients and increased correlated with infection of EBNA1 IgG.