Sucrase in immobilized cells of Cucumis sativus L.

S.I. Ignatova; L.M. Kušč; L. Bilisics; L. Trinh; N.N. Tiuliaeva; M.G. Sedova

doi:10.17221/4465-hortsci

Horticultural Science (Mar 2002)

Sucrase in immobilized cells of Cucumis sativus L.

S.I. Ignatova,
L.M. Kušč,
L. Bilisics,
L. Trinh,
N.N. Tiuliaeva,
M.G. Sedova

Affiliations

S.I. Ignatova: Selection & Seed Production Agrocompany "Iljinicha, Llc", Moscow, Russia
L.M. Kušč: Selection & Seed Production Agrocompany "Iljinicha, Llc", Moscow, Russia
L. Bilisics: Selection & Seed Production Agrocompany "Iljinicha, Llc", Moscow, Russia
L. Trinh: Selection & Seed Production Agrocompany "Iljinicha, Llc", Moscow, Russia
N.N. Tiuliaeva: Selection & Seed Production Agrocompany "Iljinicha, Llc", Moscow, Russia
M.G. Sedova: Selection & Seed Production Agrocompany "Iljinicha, Llc", Moscow, Russia

DOI: https://doi.org/10.17221/4465-hortsci
Journal volume & issue: Vol. 29, no. 1
pp. 17 – 22

Abstract

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Cell suspension cultures of Cucumis sativus L. - cucumber were permeabilized by Tween, hexadecyltri-methylammonium bromide, hexadecylpyridiniumbromide ethanol and/or immobilized by glutaraldehyde. The highest invertase activity was at pH 4.4 and temperature 53°C. The hydrolysis of the substrate was linear for 5 h reaching 60% conversion. The cells displayed high sucrase activity and convenient physico-mechanical properties.

Published in Horticultural Science

ISSN: 0862-867X (Print); 1805-9333 (Online)
Publisher: Czech Academy of Agricultural Sciences
Country of publisher: Czechia
LCC subjects: Agriculture: Agriculture (General)
Website: https://hortsci.agriculturejournals.cz/

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