PLoS ONE (Jan 2013)

Induced sputum MMP-1, -3 & -8 concentrations during treatment of tuberculosis.

  • Cesar A Ugarte-Gil,
  • Paul Elkington,
  • Robert H Gilman,
  • Jorge Coronel,
  • Liku B Tezera,
  • Antonio Bernabe-Ortiz,
  • Eduardo Gotuzzo,
  • Jon S Friedland,
  • David A J Moore

DOI
https://doi.org/10.1371/journal.pone.0061333
Journal volume & issue
Vol. 8, no. 4
p. e61333

Abstract

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IntroductionTuberculosis (TB) destroys lung tissues and this immunopathology is mediated in part by Matrix Metalloproteinases (MMPs). There are no data on the relationship between local tissue MMPs concentrations, anti-tuberculosis therapy and sputum conversion.Materials and methodsInduced sputum was collected from 68 TB patients and 69 controls in a cross-sectional study. MMPs concentrations were measured by Luminex array, TIMP concentrations by ELISA and were correlated with a disease severity score (TBscore). 46 TB patients were then studied longitudinally at the 2nd, 8th week and end of treatment.ResultsSputum MMP-1,-2,-3,-8,-9 and TIMP-1 and -2 concentrations are increased in TB. Elevated MMP-1 and -3 concentrations are independently associated with higher TB severity scores (pConclusionsMMPs are elevated in TB patients and associate with disease severity. This matrix-degrading phenotype resolves rapidly with treatment. The MMP profile at presentation correlates with a delayed treatment response.