Journal of Krishna Institute of Medical Sciences University (Jan 2020)
Post Oral Administration of Epigallocatechin Gallate from Camelia sinensis Extract Enhances Vascular Endothelial Growth Factor and Fibroblast Growth Factor Expression during Orthodontic Tooth Movement in Wistar Rats
Abstract
Background:East Java green tea leaf (Camelia sinensis) possesed active compound such as Epigallocatechin Gallate (EGCG) is well known for enhancing the bone remodelling through enhancement of Vascular Endothelial Growth Factor (VEGF) and Fibroblast Growth Factors (FGF-2). Remodelling of alveolar bone is very important to obtain optimal Orthodontic Tooth Movement (OTM) to align the tooth. Aim: To investigate the expression of VEGF and FGF-2 expression during OTM in Wistar rat after administration of EGCG from C. sinensis Extract (EGCG-CSE) Wistar rats. Material and Methods: This study was true experimental study with post-test only control group design. Twenty eight Wistar rats were randomly selected and divided into four groups accordingly; K- group which did not get both EGCGCSE administration and OTM; K+ group with OTM for 14 days, but no EGCG-CSE administration; 1 (T1) with 4 days of OTM and 7 days of EGCG-CSE administration; treatment group 2 (T2) with both 14 days OTM and EGCG-CSE administration. Ten g force/mm2 of NiTi close coil spring was installed between the upper left molars and cental insicive to move the molar mesially that induce OTM. All OTM animal model were terminated in the 14th days. Maxillary was isolated for immunohistochemistry investigation. Tukey Honest Significant Difference (HSD) was done after Analysis of Variance (ANOVA) test to investigate the significant difference between groups (p<0.05). Results: The highest positive VEGF expression was found in the T2 in both area. Meanwhile, the highest positive FGF-2 expression was found in the K-group in both area. There were significant different of VEGF and FGF-2 expression in both area between groups except T1 and T2. Conclusion: Post administration of EGCG-CSE can stimulate the VEGF and FGF-2 expression during OTM in Wistar rats.
