ZnO nanostructure-based electrochemical biosensor for Trichinella DNA detection

Abstract

A DNA electrochemical sensor's construction based on ZnO nanostructured electrodes is described in the article. As object of the study, DNA primers and PCR products of Trichinella britovi and Trichinella spiralis were chosen. The sensors were prepared with different morphologies of ZnO structures on the surfaces of the working electrodes: thin film of zinc oxide, nanorods and nanotubes. Immobilization processes of DNA primers were studied. Differential Pulse Voltammetry was used as a method of measurements of electrochemical characteristics. The measurements have shown an eligible difference of signals for confident distinction of Trichinella britovi and Trichinella spiralis PCR products. Keywords: DNA electrochemical sensor, ZnO nanostructures, Trichinella, DNA hybridization, DPV