The Journal of Engineering (Nov 2019)
Photoelectric detection of crop fungi resistance based on microfluidic
Abstract
In view of the defects such as long time-consuming and large limitation in the detection of pesticide resistance of crop pathogens at present. A novel photoelectric detection method based on enriched microfluidic chips is proposed for the detection of pesticide resistance of crop fungal disease. First, according to the aerodynamics characteristics of microbial aerosol particles and optical detection technology, a special enrichment microfluidic chip was designed to achieve high efficiency enrichment and high precision concentration detection of fungal spores. On this basis, based on the theory of pesticide resistance sensitivity baseline, the relationship among spore germination, systematic enrichment concentration, and optical detection concentration of Botrytis cinerea in strawberry treated with Pyrimethanil was studied, and the pesticide resistance detection model of uncultured pathogens was established. The light intensity of light source was 1.1 × 10^3 cd, and the wavelength was 620 nm as the optimum parameters for optical detection. The correlation coefficient between the microscopic examination of spores’ concentration and the results of optical detection is 0.9792. It has good linearity and reliability, which provides a theoretical basis for the research and development of portable equipment for detecting pesticide resistance of crop diseases.
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