Induction of gynogenesis by heterogenous sperm and cold shock treatment in Epinephelus fuscoguttatus

Weiwei Zhang; Jiawei Fan; Xin Wen; Xin Fan; Yesong Liang; Jing He; Yanqiang Li; Shuailong Chen; Mengmeng Chen; Guangcan Wu; Jian Luo

Reproduction and Breeding (Sep 2021)

Induction of gynogenesis by heterogenous sperm and cold shock treatment in Epinephelus fuscoguttatus

Weiwei Zhang,
Jiawei Fan,
Xin Wen,
Xin Fan,
Yesong Liang,
Jing He,
Yanqiang Li,
Shuailong Chen,
Mengmeng Chen,
Guangcan Wu,
Jian Luo

Affiliations

Weiwei Zhang: State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Aquaculture Breeding Engineering Research Center, Hainan Academician Team Innovation Center, Hainan University, Haikou, 570228, China
Jiawei Fan: State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Aquaculture Breeding Engineering Research Center, Hainan Academician Team Innovation Center, Hainan University, Haikou, 570228, China
Xin Wen: State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Aquaculture Breeding Engineering Research Center, Hainan Academician Team Innovation Center, Hainan University, Haikou, 570228, China
Xin Fan: State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Aquaculture Breeding Engineering Research Center, Hainan Academician Team Innovation Center, Hainan University, Haikou, 570228, China
Yesong Liang: State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Aquaculture Breeding Engineering Research Center, Hainan Academician Team Innovation Center, Hainan University, Haikou, 570228, China
Jing He: State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Aquaculture Breeding Engineering Research Center, Hainan Academician Team Innovation Center, Hainan University, Haikou, 570228, China
Yanqiang Li: State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Aquaculture Breeding Engineering Research Center, Hainan Academician Team Innovation Center, Hainan University, Haikou, 570228, China
Shuailong Chen: State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Aquaculture Breeding Engineering Research Center, Hainan Academician Team Innovation Center, Hainan University, Haikou, 570228, China
Mengmeng Chen: State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Aquaculture Breeding Engineering Research Center, Hainan Academician Team Innovation Center, Hainan University, Haikou, 570228, China
Guangcan Wu: State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Aquaculture Breeding Engineering Research Center, Hainan Academician Team Innovation Center, Hainan University, Haikou, 570228, China
Jian Luo: Corresponding author. State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, Hainan, PR China.; State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Aquaculture Breeding Engineering Research Center, Hainan Academician Team Innovation Center, Hainan University, Haikou, 570228, China

Journal volume & issue: Vol. 1, no. 3
pp. 174 – 181

Abstract

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Gynogenesis is a chromosome set manipulation technique to accelerate inbred lines used for genomics analysis and genetic breeding. Groupers are hermaphroditic fish whereby they sexually mature as females and subsequently change sex to male. Production of the homozygous inbred lines of groupers will take a prolonged period with the traditional method. This study presents the meiotic gynogenesis induction of the commercially cultured grouper Epinephelus fuscoguttatus, using a combination of ultraviolet-irradiated heterogenous sperm of E. lanceolatus and recovering second polar body by cold-shock treatment. DNA ploidy verification by flow cytometry showed that the haploid larvae exhibited abnormalities and diploids developed normally. The results indicated that UV irradiation doses at 150 mJ/cm2 produced 100% haploid larvae. The high percentage (52.1% ± 8.50%) of gynogenetic diploidy was induced by cold shock at 5–7 °C for 10 min, initiated 6 min after fertilization. The gynogenetic offspring presented 48 chromosomes (2n = 48t) and were morphologically identical to maternal chromosomes. Cytological and morphological evidence suggested that gynogenetic individuals were successfully obtained. Histological examination revealed that the gynogens gonads could develop into ovaries. Summarily, the gynogenesis technique was very representative of creating inbred lines in groupers. The successful production of this inbred lines is of great significance in grouper genetic breeding and genomic analysis.

Published in Reproduction and Breeding

ISSN: 2667-0712 (Online)
Publisher: KeAi Communications Co. Ltd.
Country of publisher: China
LCC subjects: Science: Biology (General): Genetics; Science: Biology (General): Reproduction; Science: Physiology: Animal biochemistry
Website: https://www.keaipublishing.com/en/journals/reproduction-and-breeding/

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