COMPARISON OF SEROLOGICAL INTERFERENCE FOLLOWING VACCINATION WITH BRUCELLA AB~RTUS STRAINS I N ROUTINE DIAGNOSTIC TESTS

Abstract

The serological interference following Brucella aborfus SR851 and S99 vaccination, in routine diagnostic tests was evaluafed in catf/e of four months and above. Sero negative animals were grouped into three. All the six animals of group 1 and /1 were subcutaneously vaccinated with 1.8x 1010 CFU and 1 x 1010 CFU of Brucella abortus SR851 respectively, whereas aU the.. group ll! animals (6 numbers) were vaccinated with 4 x1010 CFU of Brucella abortus S19 subcutaneously. Blood samples were collected from all animals on days D, 7, 14, 21, 28, 60, 90, 120, 950 and 180 following vaccination. Antibodies to S-LPS were measured in all three groups of animals by RBPT and STAT. None of the Brucella abortus SRB51 vaccinated animals, both with higher or lower dose, produced detectable antibody response in the conventional serological tests viz., RBPT and STAT fhroughout the study period, whereas animals vaccinated with Brucella abortus S19 produced detectable antibody responses in RBPT and STAT.

Keywords

• brucella abortus srb51
• brucella abortus strain 19
• serological tests
• interference