Enhancement of grain number per spike by RNA interference of cytokinin oxidase 2 gene in bread wheat
Yulian Li,
Guoqi Song,
Jie Gao,
Shujuan Zhang,
Rongzhi Zhang,
Wei Li,
Mingli Chen,
Min Liu,
Xianchun Xia,
Thierry Risacher,
Genying Li
Affiliations
Yulian Li
Crop Research Institute, Shandong Academy of Agricultural Sciences; Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture, National Engineering Laboratory for Wheat and Maize
Guoqi Song
Crop Research Institute, Shandong Academy of Agricultural Sciences; Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture, National Engineering Laboratory for Wheat and Maize
Jie Gao
Crop Research Institute, Shandong Academy of Agricultural Sciences; Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture, National Engineering Laboratory for Wheat and Maize
Shujuan Zhang
Crop Research Institute, Shandong Academy of Agricultural Sciences; Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture, National Engineering Laboratory for Wheat and Maize
Rongzhi Zhang
Crop Research Institute, Shandong Academy of Agricultural Sciences; Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture, National Engineering Laboratory for Wheat and Maize
Wei Li
Crop Research Institute, Shandong Academy of Agricultural Sciences; Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture, National Engineering Laboratory for Wheat and Maize
Mingli Chen
Crop Research Institute, Shandong Academy of Agricultural Sciences; Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture, National Engineering Laboratory for Wheat and Maize
Min Liu
Crop Research Institute, Shandong Academy of Agricultural Sciences; Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture, National Engineering Laboratory for Wheat and Maize
Xianchun Xia
Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS)
Thierry Risacher
Biogemma, Site de la Garenne
Genying Li
Crop Research Institute, Shandong Academy of Agricultural Sciences; Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture, National Engineering Laboratory for Wheat and Maize
Abstract Background This study aimed to validate the function of CKX gene on grain numbers in wheat. Methods we constructed and transformed a RNA interference expression vector of TaCKX2.4 in bread wheat line NB1. Southern blotting analysis was used to select transgenic plants with single copy. The expression of TaCKX2.4 gene was estimated by Quantitative real-time PCR (qRT-PCR) analysis. Finally, the relation between expression of TaCKX2.4 gene and grain numbers was validated. Results Totally, 20 positive independent events were obtained. Homozygous lines from 5 events with a single copy of transformed gene each were selected to evaluate the expression of TaCKX2.4 and grain numbers per spike in T3 generation. Compared with the control NB1, the average grain numbers per spike significantly increased by 12.6%, 8.3%, 6.5% and 5.8% in the T3 lines JW39-3A, JW1-2B, JW1-1A and JW5-1A, respectively. Conclusion Our study indicated that the expression level of TaCKX2.4 was negatively correlated with the grain number per spike, indicating that the reduced expression of TaCKX2.4 increased grain numbers per spike in wheat.
