Prion-Seeding Activity Is widely Distributed in Tissues of Sporadic Creutzfeldt-Jakob Disease Patients

Hanae Takatsuki, PhD; Takayuki Fuse, PhD; Takehiro Nakagaki, MD, PhD; Tsuyoshi Mori, PhD; Ban Mihara, MD, PhD; Masaki Takao, MD, PhD; Yasushi Iwasaki, MD, PhD; Mari Yoshida, MD, PhD; Shigeo Murayama, MD, PhD; Ryuichiro Atarashi, MD, PhD; Noriyuki Nishida, MD, PhD; Katsuya Satoh, MD, PhD

doi:10.1016/j.ebiom.2016.08.033

EBioMedicine (Oct 2016)

Prion-Seeding Activity Is widely Distributed in Tissues of Sporadic Creutzfeldt-Jakob Disease Patients

Hanae Takatsuki, PhD,
Takayuki Fuse, PhD,
Takehiro Nakagaki, MD, PhD,
Tsuyoshi Mori, PhD,
Ban Mihara, MD, PhD,
Masaki Takao, MD, PhD,
Yasushi Iwasaki, MD, PhD,
Mari Yoshida, MD, PhD,
Shigeo Murayama, MD, PhD,
Ryuichiro Atarashi, MD, PhD,
Noriyuki Nishida, MD, PhD,
Katsuya Satoh, MD, PhD

Affiliations

Hanae Takatsuki, PhD: Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Takayuki Fuse, PhD: Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Takehiro Nakagaki, MD, PhD: Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Tsuyoshi Mori, PhD: Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan
Ban Mihara, MD, PhD: Department of Neurology, Institute of Brain and Blood Vessels, Mihara Memorial Hospital, Isesaki, Japan
Masaki Takao, MD, PhD: Department of Neurology, Institute of Brain and Blood Vessels, Mihara Memorial Hospital, Isesaki, Japan
Yasushi Iwasaki, MD, PhD: Department of Neuropathology, Institute for Medical Science of Aging, Aichi Medical University, Aichi, Japan
Mari Yoshida, MD, PhD: Department of Neuropathology, Institute for Medical Science of Aging, Aichi Medical University, Aichi, Japan
Shigeo Murayama, MD, PhD: Department of Neuropathology, Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology, Tokyo, Japan
Ryuichiro Atarashi, MD, PhD: Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan
Noriyuki Nishida, MD, PhD: Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Katsuya Satoh, MD, PhD: Department of Locomotive Rehabilitation Science, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan

DOI: https://doi.org/10.1016/j.ebiom.2016.08.033
Journal volume & issue: Vol. 12, no. C
pp. 150 – 155

Abstract

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Human prion diseases are neurodegenerative disorders caused by abnormally folded prion proteins in the central nervous system. These proteins can be detected using the quaking-induced conversion assay. Compared with other bioassays, this assay is extremely sensitive and was used in the present study to determine prion distribution in sporadic Creutzfeldt-Jakob disease patients at autopsy. Although infectivity of the sporadic form is thought to be restricted within the central nervous system, results showed that prion-seeding activities reach 106/g from a 50% seeding dose in non-neuronal tissues, suggesting that prion-seeding activity exists in non-neural organs, and we suggested that non-neural tissues of 106/g SD50 did not exist the infectivity.

Published in EBioMedicine

ISSN: 2352-3964 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General)
Website: http://www.journals.elsevier.com/ebiomedicine/

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