Evaluation of Wild Peanut Species and Their Allotetraploids for Resistance against Thrips and Thrips-Transmitted Tomato Spotted Wilt Orthotospovirus (TSWV)

Yi-Ju Chen; Sudeep Pandey; Michael Catto; Soraya Leal-Bertioli; Mark R. Abney; Sudeep Bag; Mark Hopkins; Albert Culbreath; Rajagopalbabu Srinivasan

doi:10.3390/pathogens12091102

Pathogens (Aug 2023)

Evaluation of Wild Peanut Species and Their Allotetraploids for Resistance against Thrips and Thrips-Transmitted Tomato Spotted Wilt Orthotospovirus (TSWV)

Yi-Ju Chen,
Sudeep Pandey,
Michael Catto,
Soraya Leal-Bertioli,
Mark R. Abney,
Sudeep Bag,
Mark Hopkins,
Albert Culbreath,
Rajagopalbabu Srinivasan

Affiliations

Yi-Ju Chen: Department of Entomology, University of Georgia, Griffin, GA 30223, USA
Sudeep Pandey: Department of Entomology, University of Georgia, Griffin, GA 30223, USA
Michael Catto: Department of Entomology, University of Georgia, Athens, GA 30602, USA
Soraya Leal-Bertioli: Department of Plant Pathology, Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, GA 30602, USA
Mark R. Abney: Department of Entomology, University of Georgia, Tifton, GA 31794, USA
Sudeep Bag: Department of Plant Pathology, University of Georgia, Tifton, GA 31793, USA
Mark Hopkins: Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, GA 30602, USA
Albert Culbreath: Department of Plant Pathology, University of Georgia, Tifton, GA 31793, USA
Rajagopalbabu Srinivasan: Department of Entomology, University of Georgia, Griffin, GA 30223, USA

DOI: https://doi.org/10.3390/pathogens12091102
Journal volume & issue: Vol. 12, no. 9
p. 1102

Abstract

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Thrips-transmitted tomato spotted wilt orthotospovirus (TSWV) causes spotted wilt disease in peanut (Arachis hypogaea L.) and limits yield. Breeding programs have been developing TSWV-resistant cultivars, but availability of sources of resistance against TSWV in cultivated germplasm is extremely limited. Diploid wild Arachis species can serve as important sources of resistance, and despite ploidy barriers (cultivated peanut is tetraploid), their usage in breeding programs is now possible because of the knowledge and development of induced interspecific allotetraploid hybrids. This study screened 10 wild diploid Arachis and six induced allotetraploid genotypes via thrips-mediated TSWV transmission assays and thrips’ feeding assays in the greenhouse. Three parameters were evaluated: percent TSWV infection, virus accumulation, and temporal severity of thrips feeding injury. Results indicated that the diploid A. stenosperma accession V10309 and its derivative-induced allotetraploid ValSten1 had the lowest TSWV infection incidences among the evaluated genotypes. Allotetraploid BatDur1 had the lowest thrips-inflicted damage at each week post thrips release, while diploid A. batizocoi accession K9484 and A. duranensis accession V14167 had reduced feeding damage one week post thrips release, and diploids A. valida accession GK30011 and A. batizocoi had reduced feeding damage three weeks post thrips releasethan the others. Overall, plausible TSWV resistance in diploid species and their allotetraploid hybrids was characterized by reduced percent TSWV infection, virus accumulation, and feeding severity. Furthermore, a few diploids and tetraploid hybrids displayed antibiosis against thrips. These results document evidence for resistance against TSWV and thrips in wild diploid Arachis species and peanut-compatible-induced allotetraploids.

Published in Pathogens

ISSN: 2076-0817 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine
Website: http://www.mdpi.com/journal/pathogens

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