Effects of Feeding Low Protein Diets on Serum and Faeces Parameters in Weaned Piglets

Abstract

This study was conducted to determine the effects of a low-protein diet supplemented with synthetic amino acids on the biochemical parameters in the blood serum, the indicators of fermentation processes, and nitrogen excretion in 12 crossbred piglets. The piglets (weaned at 28 days of age) were divided into two groups with 6 piglets each. The control group had an initial average body weight of 8.8 ± 0.6 kg and the experimental group with an average initial body weight of 8.6 ± 0.7 kg. The control diet contained 210.8 g.kg−1 crude protein and the experimental diet contained 186.4 g.kg−1. The experimental diet was supplemented with lysine, methionine and threonine to achieve a more ideal amino acid pattern. The blood collections from the sinus ophthalmicus for the determination of the biochemical parameters were performed 4 times at weekly intervals in the control and experimental groups 4—5 hours after feeding. The faeces were taken from the rectum at the end of the study period. The decrease in the dietary crude protein content of the experimental group was manifested by a significant decrease of the blood urea level (2.61 mmol.l−1 average concentration) compared to the control groups (4.21 mmol.l−1 average concentration) (P < 0.001). The other serum component concentrations (total protein, albumin, glucose, cholesterol, total lipids and selected enzymes) showed no significant statistical changes between the control and experimental groups. The results of the fermentation process analysis indicated that the butyrate concentration decreased (P = 0.0017) and the pH increased (P = 0.0180) in the experimental group compared to the control group. The levels of crude protein and ammonia in the faeces of experimental animals were significantly lower (P < 0.001) in comparison with those in the control animals.

Keywords

• amino acids
• ammonia
• crude protein
• fermentation
• metabolism
• pig
• volatile fatty acids