Generation of induced pluripotent stem cell Line KAIMRCi001-A by reprogramming erythroid progenitors from peripheral blood of a healthy Saudi donor

Mohammad Al-Shehri; Moayad Baadhaim; Shereen Jamalalddin; Doaa Aboalola; Mustafa Daghestani; Hajar AlZahrani; Dalal Malibari; Mohammad Mubaraki; Kholoud Aldubayan; Mohammed AlBalwi; Khaled Alsayegh

Stem Cell Research (Oct 2021)

Generation of induced pluripotent stem cell Line KAIMRCi001-A by reprogramming erythroid progenitors from peripheral blood of a healthy Saudi donor

Mohammad Al-Shehri,
Moayad Baadhaim,
Shereen Jamalalddin,
Doaa Aboalola,
Mustafa Daghestani,
Hajar AlZahrani,
Dalal Malibari,
Mohammad Mubaraki,
Kholoud Aldubayan,
Mohammed AlBalwi,
Khaled Alsayegh

Affiliations

Mohammad Al-Shehri: King Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard – Health Affairs, Jeddah, Saudi Arabia
Moayad Baadhaim: King Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard – Health Affairs, Jeddah, Saudi Arabia
Shereen Jamalalddin: College of Science and Health Professions, King Saud Bin Abdulaziz University for Health Sciences, Jeddah, Saudi Arabia
Doaa Aboalola: King Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard – Health Affairs, Jeddah, Saudi Arabia
Mustafa Daghestani: King Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard – Health Affairs, Jeddah, Saudi Arabia; Department of Pathology and Laboratory Medicine, Ministry of the National Guard-Health Affairs, Jeddah Saudi Arabia
Hajar AlZahrani: King Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard – Health Affairs, Jeddah, Saudi Arabia
Dalal Malibari: King Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard – Health Affairs, Jeddah, Saudi Arabia
Mohammad Mubaraki: Department of Pathology and Laboratory Medicine, King Abdulaziz Medical City (KAMC), MNGHA, Riyadh 11426, Saudi Arabia; King Abdullah International Medical Research Center, Department of Medical Genomic Research, King Saud Bin Abdulaziz University for Health Sciences, Riyadh 11481, Saudi Arabia
Kholoud Aldubayan: Department of Pathology and Laboratory Medicine, King Abdulaziz Medical City (KAMC), MNGHA, Riyadh 11426, Saudi Arabia; King Abdullah International Medical Research Center, Department of Medical Genomic Research, King Saud Bin Abdulaziz University for Health Sciences, Riyadh 11481, Saudi Arabia
Mohammed AlBalwi: Department of Pathology and Laboratory Medicine, King Abdulaziz Medical City (KAMC), MNGHA, Riyadh 11426, Saudi Arabia; King Abdullah International Medical Research Center, Department of Medical Genomic Research, King Saud Bin Abdulaziz University for Health Sciences, Riyadh 11481, Saudi Arabia; College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, Riyadh 11481, Saudi Arabia
Khaled Alsayegh: King Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard – Health Affairs, Jeddah, Saudi Arabia; Corresponding author.

Journal volume & issue: Vol. 56
p. 102548

Abstract

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In this study we isolated and enriched erythroid progenitor cells (EPCs) from a 10 ml peripheral blood sample from a 37-year old healthy Saudi donor. After expansion, these EPCs were reprogrammed using episomal plasmids to generate an induced pluripotent stem (iPS) cell line, KAIMRCi001-A. The pluripotency of this line was confirmed by measuring the expression of typical pluripotency markers and assessing differentiation potential in vitro.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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