Clinical Utility of Molecular Diagnostics in Children with Respiratory Infections

Abstract

Objective: Lower respiratory tract infections (LRTI) are among the major causes of mortality in children worldwide. Our study aimed to determine viral agents in children under five years of age who were followed up with acute LRTI. Methods: Nasopharyngeal swab samples were taken from children aged 1 month to 5 years who were diagnosed with LRTI at the Bezmialem Vakıf University pediatric emergency department between March 1, 2015 and January 31, 2016. Cases with underlying chronic diseases were excluded from the study. The presence of the agent was investigated in the samples taken using the real-time polymerase chain reaction method. The distribution of cases in which the agent was detected according to age groups and seasons was examined. The relationship between the agent and the clinical and laboratory findings was investigated. Results: Of the 95 patients included in the study, 51 (53.7%) were male, 44 (46.3%) were female, and the mean age was 26.2 months. The presence of viruses was shown in 50 cases (52.6%). The most frequently detected viruses were rhinovirus (28%), human bocavirus (HBoV) (26%) and respiratory syncytial virus (RSV) (24%). While RSV infections were more common in winter months, HBoV was observed to persist throughout the year. No significant relationship was found between the clinical and laboratory findings of the patients and the agents. Conclusion: Viral etiology was detected in 52.6% of the cases. Molecular tests are valuable in detecting more than one virus in the distribution of viruses among different age groups.

Keywords

• infections
• respiratory tract
• multiplex polymerase chain reaction