Infection and Drug Resistance (Apr 2022)
Analysis of Bactericidal Effect of Three Medical Ozonation Dosage Forms on Multidrug-Resistant Bacteria from Burn Patients
Abstract
Xuan Wang,1,* Dan Liao,2,* Qiu-Ming Ji,2 Yu-Hong Yang,2 Ming-Chao Li,3 Xian-Yun Yi,2 Chi Li,2 Yu Chen,2 Hong-Bo Tao,2 Wen-Hui Zhai2 1Institute of Burns, Wuhan Third Hospital, Wuhan University, Wuhan, 430000, People’s Republic of China; 2Department of Psychiatry, Wuhan Wudong Hospital, Wuhan, 430084, People’s Republic of China; 3Department of Psychiatry, Affiliated Wuhan Mental Health Center, Tongji Medical College of Huazhong University Science & Technology, Wuhan, 430022, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qiu-Ming Ji; Yu-Hong Yang, Department of Psychiatry, Wuhan Wudong Hospital, No. 46 of Wudong Street, Qingshan District, Wuhan, 430084, People’s Republic of China, Tel +86 27 5052 8367, Fax +86 27 8643 8247, Email [email protected]; [email protected]: To examine the bactericidal effects of three different states of medical ozone (liquid, gas, and oil) against drug-resistant strains of common bacteria on burn wounds, which could as a clinical reference.Methods: Three multidrug-resistant strains of methicillin-resistant Staphylococcus aureus, pan-resistant Pseudomonas aeruginosa, and ESBLs Klebsiella pneumoniae were identified from burn wounds. The colonies of the three varieties of bacteria were each carried out using the pour plate method prior to the start of the experiment. Then, depending on the state of ozone, different treatment procedures are applied. Group of ozone gas: in a closed glass jar, the bacterial liquid was injected into a single layer of sterile gauze, and the ozone gas concentration was held at 50 g/mL. The bacterial liquid was diluted and combined directly with ozone water in the ozone water group. Ozone is a type of oil: after the emulsifier was added to the oil group. The gas, water, and oil groups were rapidly neutralized and counted again after 5, 10, and 30 minutes.Results: Ozone gas and oil groups totally eliminated multidrug resistant bacteria in the above study within 30 minutes. (2) At 5 and 10 minutes, the difference in bactericidal effect between ozone gas group and ozone water and oil group was statistically significant (P 0.05); at the time of 30 minutes, the effects of bactericidal effect between ozone water group and ozone gas and oil had no significance (P> 0.05).Conclusion: Ozone has the ability to kill bacteria, depending on the treatment time, different ozone types should be chosen for sterilization and disinfection in clinical application.Keywords: ozone, burn, wound treatment, multidrug resistant bacteria
