Nature Communications (Feb 2018)

Single-cell RNA-seq of rheumatoid arthritis synovial tissue using low-cost microfluidic instrumentation

  • William Stephenson,
  • Laura T. Donlin,
  • Andrew Butler,
  • Cristina Rozo,
  • Bernadette Bracken,
  • Ali Rashidfarrokhi,
  • Susan M. Goodman,
  • Lionel B. Ivashkiv,
  • Vivian P. Bykerk,
  • Dana E. Orange,
  • Robert B. Darnell,
  • Harold P. Swerdlow,
  • Rahul Satija

DOI
https://doi.org/10.1038/s41467-017-02659-x
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 10

Abstract

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Droplet-based single-cell RNA-seq is a powerful tool for cellular heterogeneity profiling in disease but is limited by instrumentation required. Here the authors develop a 3D printed microfluidic platform for massive parallel sequencing of rheumatoid arthritis tissues.