Hydrolyzed VCO Cream Reduces Neutrophil Number and Increases Angiogenesis in Mid Dermal Burn Wound Healing

Abstract

BACKGROUND: Researches on plant active substances are conducted to find effective topical drugs for mid dermal burns wound. The 70% hydrolyzed VCO (hVCO) cream has been known to accelerate macroscopic healing of mid dermal burns compared to 30% and 50% hVCO creams. This study was conducted to further investigate the effectiveness of 70% hVCO cream and find the most effective doses of hVCO to heal mid dermal burns based on the macroscopic diameter, neutrophil number and angiogenesis. METHODS: Thirty mid dermal burns-induced male Rattus norvegicus were randomly divided into 6 groups that were either treated with 70% hVCO cream, 100% hVCO cream, or basic cream for 6 or 12 days. Macroscopic appearance of mid dermal burn was documented, and the diameter was calculated. Hematoxylin Eosin (HE) examination was conducted to measure the neutrophil and angiogenesis in mid dermal burn wound. RESULTS: The decrease of wound macroscopic size was found to be significant after 6 and 12 days of treatment (p=0.004 and p<0.001, respectively), with the smallest diameter was found in 70% hVCO group. The lowest number of neutrophils was found in the 70% hVCO group at day-6 (p=0.039) and day-12 (p=0.013). There was a significant increase of angiogenesis at day-12 (p=0.025) with the highest value obtained in the 70% hVCO group. CONCLUSION: The 70% hVCO decreases the wound macroscopic size, decreases neutrophil number, and increases angiogenesis compared to the 100% hVCO. Hence, 70% hVCO isshown to be the most effective dose in accelerating mid dermal burns wound healing andcan be explored further as topical medication. KEYWORDS: hVCO, neutrophil, angiogenesis, wound healing