Инфекция и иммунитет (Dec 2015)

MICROBIAL LANDSCAPE OF MICROFLORA OF A PHARYNX AT PATIENTS WITH TONSILLIT’S PATHOLOGY

  • O. Yu. Borisova,
  • V. A. Aleshkin,
  • A. S. Pimenova,
  • A. I. Krukov,
  • N. L. Kunelskaya,
  • A. V. Gurov,
  • G. B. Shadrin,
  • A. S. Tovmasyan,
  • B. A. Efimov,
  • L. I. Kafarskaya

DOI
https://doi.org/10.15789/2220-7619-2015-3-225-232
Journal volume & issue
Vol. 5, no. 3
pp. 225 – 232

Abstract

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The microbial landscape of microflora of a pharynx at patients with tonsillit’s pathology were studied. Materials and methods. 79 patients from GBUZ “Research Institute of Clinical Otorhinolaryngology” (78.5% patients with various forms of chronic tonsillitis and 21.5% of patients without chronic tonsillitis (control group). Among patients of the main group with chronic tonsillitis, at 60 (96.8%) patients there was a diagnosis chronic tonsillitis a toxical-allergic form of 1 degree (TAF I), at 1 (1.6%) the patient — chronic tonsillitis a toxical-allergic form II of degree (TAF II) and at 1 (1.6%) the patient — chronic tonsillitis. Identification of microorganisms carried out on cultural-morphological and biochemical properties. Specific identification of the hardly cultivated microorganisms and Corynebacterium was carried out by MALDI-TOFF MS of BioMerieux VITEK MS MALDI-TOF (“bioMerieux”, France). Identification of the allocated Corynebacterium was carried out by amplification of a gene rpoB and the subsequent direct sequencing. Results. The majority (98.7%) of the allocated microorganisms, treated 27 types and were Gram-positive. It is revealed 159 strains of 29 species of microorganisms, from them 41.4% of strains belonged to the Streptococcus, 19.7% — Staphylococcus, 36.9% — Corynebacterium. Among Streptococcus — 55.4% of S. viridans, 38.4% — S. pyogenes and 3.1% — of S. pneumoniae и S. oralis; Staphylococcus — 64.5% of S. aureus, 32.2% of S. epidermidis and 3.3% of S. hominis. 18 types of Corynebacterium — С. tuberculostearicum (17.2% strains), C. рseudodiphtheriticum (15.5% strains) and C. aurimucosum (18.9% strains) are revealed. At 44.3% of the surveyed patients the microflora is presented by a monoculture and at 55.7% associations are revealed. Conclusion. The microflora at patients with tonsillit’s pathology is characterized. At the expressed pathological process in a microbiota of a pharynx the monoculture while existence of associations testifies to less expressed pathological process prevails.

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