مجلة التربية والعلم (Jun 2009)

Isolation of leptin hormone from plasma and molecular weight estimation using bio techniques

  • Nawal Thnoon Youis,
  • Qusi Al Jalabi,
  • Thikra Ali

DOI
https://doi.org/10.33899/edusj.2009.57654
Journal volume & issue
Vol. 22, no. 2
pp. 61 – 70

Abstract

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Abstract The research include the isolation of leptin hormone from plasma of female by gel filtration chromatography technique using separating column 1002Cm containing Sephadex G - 75 produced 5 bands where number 5 showed leptin concentration 0.5ng/ml. The apparent molecular weight of leptin had been determined by gel filtration chromatography technique using Sephadex G - 75, and by SDS electrophoresis, the results showed that the apparent molecular weight for the band number 5 of leptin hormone was 15750353 Dalton and 16000 Dalton respectively.

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