Assess on rabbit conjunctival lymphatic vessels distribution in different age groups

Abstract

AIM: To investigate the distribution of conjunctival lymphatic vessels in New Zealand white rabbits and its relationship with age.METHODS: Subconjunctival trypan blue was injected in four quadrants(nasal, superior, temporal, and inferior)and recorded videos. The number of lymphatic outflow pathways arising from the blebs was counted. The difference in the number of lymphatic outflow pathways across the quadrants of each group was tested by single-factor analysis of variance. Paired t-test was used to compare the differences in the number of lymphatic outflow pathways in the same rabbit quadrants across different ages.RESULTS: In New Zealand white rabbits, thick annular lymphatic vessels were mainly distributed near the limbus part of the conjunctiva. The single collecting lymphatic vessels were primarily distributed in the peripheral parts. Although the lymphatic vessels of juvenile rabbits have fewer branches, the number of lymphatic vessels in the different quadrants was no significant difference in each group(all P>0.05)and was no difference between the two age groups(all P >0.05).CONCLUSION:The lymphatic vessels were abundant in rabbit conjunctiva. The number of lymphatic vessels in each quadrant and between two age groups had no significant difference.

Keywords

• conjunctiva lymphatic vessels
• new zealand white rabbit
• trypan blue
• age