Analysis of peroxidase isozymes and HPLC chromatographic study of paeoniflorin in Hangzhou white peony

Abstract

The peroxidase isozymes of Hangzhou white peony were isolated by polyacrylamide gel electrophoresis. Up to 2-5 active bands of peroxidase isozyme were observed in 13 lines after active dyeing, and the rate of relative migration was located in the range from 0.567 to 0.685. Based on the differences in number and location of bands the individuals were divided into 3 groups. The results of HPLC showed that paeoniflorin was the primary component in Hangzhou white peony, and only a little albiflorin with no oxypaeoniflorin was observed. The paeoniflorin content of Hangzhou white peony as detected after different processing was different, although the HPLC chromatograms of paeoniflorin were similar.

Keywords

• Hangzhou white peony
• peroxidase
• isozyme
• paeoniflorin
• HPLC