PLoS ONE (Jan 2013)

Acetylcorynoline impairs the maturation of mouse bone marrow-derived dendritic cells via suppression of IκB kinase and mitogen-activated protein kinase activities.

  • Ru-Huei Fu,
  • Yu-Chi Wang,
  • Shih-Ping Liu,
  • Ching-Liang Chu,
  • Rong-Tzong Tsai,
  • Yu-Chen Ho,
  • Wen-Lin Chang,
  • Shao-Chih Chiu,
  • Horng-Jyh Harn,
  • Woei-Cherng Shyu,
  • Shinn-Zong Lin

DOI
https://doi.org/10.1371/journal.pone.0058398
Journal volume & issue
Vol. 8, no. 3
p. e58398

Abstract

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BackgroundDendritic cells (DCs) are major modulators in the immune system. One active field of research is the manipulation of DCs as pharmacological targets to screen novel biological modifiers for the treatment of inflammatory and autoimmune disorders. Acetylcorynoline is the major alkaloid component derived from Corydalis bungeana herbs. We assessed the capability of acetylcorynoline to regulate lipopolysaccharide (LPS)-stimulated activation of mouse bone marrow-derived DCs.Methodology/principal findingsOur experimental data showed that treatment with up to 20 µM acetylcorynoline does not cause cytotoxicity in cells. Acetylcorynoline significantly inhibited the secretion of tumor necrosis factor-α, interleukin-6, and interleukin-12p70 by LPS-stimulated DCs. The expression of LPS-induced major histocompatibility complex class II, CD40, and CD86 on DCs was also decreased by acetylcorynoline, and the endocytic capacity of LPS-stimulated DCs was restored by acetylcorynoline. In addition, LPS-stimulated DC-elicited allogeneic T-cell proliferation was blocked by acetylcorynoline, and the migratory ability of LPS-stimulated DCs was reduced by acetylcorynoline. Moreover, acetylcorynoline significantly inhibits LPS-induced activation of IκB kinase and mitogen-activated protein kinase. Importantly, administration of acetylcorynoline significantly attenuates 2,4-dinitro-1-fluorobenzene-induced delayed-type hypersensitivity.Conclusions/significanceAcetylcorynoline may be one of the potent immunosuppressive agents through the blockage of DC maturation and function.