Spirulina and C-phycocyanin mitigate titanium dioxide nanoparticle-induced hematobiochemical and hepatorenal disorders through antioxidative pathway

Abstract

The present investigation is designed to evaluate the ameliorative efficacy of spirulina and its active constituent, C-phycocyanin, on hepatorenal injury induced by titanium dioxide nanoparticles (TiO2 NPs). Forty-eight male albino rats were randomly divided into six groups: control group, Spirulina treated group, C-phycocyanin treated group, TiO2 NPs treated group, TiO2 NPs+ Spirulina treated group and TiO2 NPs+ C-phycocyanin treated group. The estimation of hepatic and renal function markers and oxidative status as well as histopathologic alterations in hepatic and renal tissues were analyzed. Spirulina and C- phycocyanin treatments caused significant improvement in all hematological indices such as RBCs, Hb, HCT, and total and differential WBCs relative to the untreated TiO2 NPs group. Also, spirulina and C-phycocyanin treatments significantly alleviated hepatic enzymes and bilirubin concentrations. Oral administration of spirulina and C- phycocyanin decreased total cholesterol and triglycerides content significantly. Both Spirulina and C- phycocyanin attenuated creatinine, urea, and uric acid significantly. Furthermore, the present study revealed that spirulina and C- phycocyanin significantly increased hepatorenal antioxidants content and retard malondialdehyde (MDA) formation. Histopathological examinations displayed that both spirulina and C- phycocyanin treatments repaired mostly the abnormal architecture of hepatic and renal tissues induced by TiO2 NPs. In conclusion, the modulatory role of spirulina and C-phycocyanin may be due to their strong antioxidative effects and other active constituents of spirulina.

Keywords

• Titanium dioxide nanoparticles
• Hepatorenal damage
• Spirulina
• C-phycocyanin
• Oxidative stress