Variability in the Viral Protein Linked to the Genome of Turnip Mosaic Virus Influences Interactions with eIF(iso)4Es in Brassica rapa

Guoliang Li; Shifan Zhang; Fei Li; Hui Zhang; Shujiang Zhang; Jianjun Zhao; Rifei Sun

doi:10.5423/PPJ.OA.07.2020.0125

The Plant Pathology Journal (Feb 2021)

Variability in the Viral Protein Linked to the Genome of Turnip Mosaic Virus Influences Interactions with eIF(iso)4Es in Brassica rapa

Guoliang Li,
Shifan Zhang,
Fei Li,
Hui Zhang,
Shujiang Zhang,
Jianjun Zhao,
Rifei Sun

Affiliations

Guoliang Li: State Key Laboratory of North China Crop Improvement and Regulation, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Shifan Zhang: State Key Laboratory of North China Crop Improvement and Regulation, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Fei Li: State Key Laboratory of North China Crop Improvement and Regulation, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Hui Zhang: State Key Laboratory of North China Crop Improvement and Regulation, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Shujiang Zhang: State Key Laboratory of North China Crop Improvement and Regulation, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Jianjun Zhao: State Key Laboratory of North China Crop Improvement and Regulation, Department of Horticulture, Hebei Agricultural University, Baoding 071001, China
Rifei Sun: State Key Laboratory of North China Crop Improvement and Regulation, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China

DOI: https://doi.org/10.5423/PPJ.OA.07.2020.0125
Journal volume & issue: Vol. 37, no. 1
pp. 47 – 56

Abstract

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Plants protect against viruses through passive and active resistance mechanisms, and in most cases characterized thus far, natural recessive resistance to potyviruses has been mapped to mutations in the eukaryotic initiation factor eIF4E or eIF(iso)4E genes. Five eIF4E copies and three eIF(iso)4E copies were detected in Brassica rapa. The eIF4E and eIF(iso)4E genes could interact with turnip mosaic virus (TuMV) viral protein linked to the genome (VPg) to initiate virus translation. From the yeast two-hybrid system (Y2H) and bimolecular fluorescence complementation (BiFC) assays, the TuMV-CHN2/CHN3 VPgs could not interact with BraA.eIF4E.a/c or BraA.eIF(iso)4E.c, but they could interact with BraA.eIF(iso)4E.a in B. rapa. Further analysis indicated that the amino acid substitution L186F (nt T556C) in TuMV-UK1 VPg was important for the interaction networks between the TuMV VPg and eIF(iso)4E proteins. An interaction model of the BraA. eIF(iso)4E protein with TuMV VPg was constructed to infer the effect of the significant amino acids on the interaction of TuMV VPgs-eIF(iso)4Es, particularly whether the L186F in TuMV-UK1 VPg could change the structure of the TuMV-UK1 VPg protein, which may terminate the interaction of the BraA.eIF(iso)4E and TuMV VPg protein. This study provides new insights into the interactions between plant viruses and translation initiation factors to reveal the working of key amino acids.

Published in The Plant Pathology Journal

ISSN: 1598-2254 (Print); 2093-9280 (Online)
Publisher: Hanrimwon Publishing Company
Country of publisher: Korea, Republic of
LCC subjects: Agriculture: Plant culture
Website: http://www.ppjonline.org/main.html

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