مجله دانشگاه علوم پزشکی گرگان (Jan 2021)
Effect of Lactobacillus paracasei probiotic in Parkinson’s male rats
Abstract
Background and Objective: Parkinsonchr('39')s disease is a neurological disorder caused by the loss of dopaminergic neurons in pars compacta of substantial nigra. The most important symptoms of this disease include slow motion, lack of movement, tremor and imbalance. This study was done to determine the effect of probiotic Lactobacillus paracasei on motor disorders caused by injection of 6-hydroxy dopamine in Parkinsonchr('39')s model in male rats. Methods: In this experimental study, 48 male rats were randomly allocated into six groups. To create an animal model of Parkinsonchr('39')s disease, a black body component in male rats was injected 6-hydroxy dopamine (6-OHDA) (0.5 mg/kg) unilaterally by stereotaxic apparatus. After 3 weeks of recovery, to evaluation of degradation rate, animals were received apomorphine, intraperitoneally. In the first experiment, rats were randomly divided into Sham and Parkinsonchr('39')s (injured) groups, the Sham group were received only water and food, and the injured group received 6-hydroxy-dopamine. In the second experiment, male rats were divided into 4 groups of 8 rats, each of the 4 groups was injected 6-hydroxy-dopamine and 21 days later, they were tested for assay of apomorphine. The results were recorded, then for 28 days, the first group was taken as control. The second group of saline, the third group of milk, the fourth group of milk and probiotic were gavaged. At the end of day 28, once again, each group was subjected to rotation of apomorphine test and the rotations were recorded. Results: The behavioral analysis of saline, milk, milk plus probiotics were indicated that the group receiving probiotic Lactobacillus paracasei plus milk was significantly less apomorphine rotation test than the saline group (P<0.05). Conclusion: It seems that probiotic Lactobacillus paracasei can reduces Parkinson’s disease symptoms.
