Uji Resistansi Antibiotik Bakteri Vibrio Parahaemolyticus dari Udang Putih (Litopaneus Vannamei) Serta Identifikasi Gen Penyandi Resistan Ampisilin

Abstract

Vibriosis in white shrimp (Litopaneus vannamei) became a great fear among fish farmers in 2009 because it was thought to cause EMS (Early Mortality Syndrome). This disease causes mass mortality at a young age. Generally, vibriosis in shrimp is prevented and treated with antibiotics. Improper use of antibiotics can lead to multiple antibiotic resistance. This study was conducted to determine the level of antibiotic resistance to Vibrio parahaemolyticus bacteria in white shrimp and to identify the presence of the ampicillin resistance gene. Vibrio parahaemolyticus bacterial isolates were tested for resistance to the antibiotics ampicillin, oxytetracycline, chloramphenicol, enrofloxacin, and erythromycin. This study was performed using the Kirby-Bauer disc diffusion method with the Clinical and Laboratory Standards Institute (CLSI) tables. Resistance testing showed that 30 isolates of V parahemolyticus were sensitive to enrofloxacin (94%), and chloramphenicol (97%). The results for ampicillin showed resistance (77%). Ampicillin-resistant strains showed the presence of genes encoding BlaTEM protein resistance in 100% (out of 6 samples) of 516 bp amplicons. It can be concluded that the V parahaemolyticus isolates are ampicillin resistant to the BlaTEM gene.

Keywords

• vibrio parahaemolyticus
• antibiotic resistance
• coding gene