In vitro antibacterial and biofilm inhibitory activity of the sawdust essential oil of Tetraclinis articulata (vahl) against catheter-associated Staphylococcus aureus clinical isolates

Mohamed Achmit; Noura Aoussar; Fouad Mellouki; Rajaa Ait Mhand; María Dolores Ibáñez; María Amparo Blázquez; Mohamed Akssira; Khalid Zerouali; Naima Rhallabi

Current Research in Biotechnology (Jan 2021)

In vitro antibacterial and biofilm inhibitory activity of the sawdust essential oil of Tetraclinis articulata (vahl) against catheter-associated Staphylococcus aureus clinical isolates

Mohamed Achmit,
Noura Aoussar,
Fouad Mellouki,
Rajaa Ait Mhand,
María Dolores Ibáñez,
María Amparo Blázquez,
Mohamed Akssira,
Khalid Zerouali,
Naima Rhallabi

Affiliations

Mohamed Achmit: Research Unit of Microbiology and Bioactive Molecules, Laboratory of Virology, Microbiology, Quality, Biotechnology, Eco-toxicology and Biodiversity, Faculty of Sciences and Techniques, P.B. 146, Mohammedia, Hassan II University of Casablanca, Morocco; Corresponding author.
Noura Aoussar: Research Unit of Microbiology and Bioactive Molecules, Laboratory of Virology, Microbiology, Quality, Biotechnology, Eco-toxicology and Biodiversity, Faculty of Sciences and Techniques, P.B. 146, Mohammedia, Hassan II University of Casablanca, Morocco
Fouad Mellouki: Research Unit of Microbiology and Bioactive Molecules, Laboratory of Virology, Microbiology, Quality, Biotechnology, Eco-toxicology and Biodiversity, Faculty of Sciences and Techniques, P.B. 146, Mohammedia, Hassan II University of Casablanca, Morocco
Rajaa Ait Mhand: Research Unit of Microbiology and Bioactive Molecules, Laboratory of Virology, Microbiology, Quality, Biotechnology, Eco-toxicology and Biodiversity, Faculty of Sciences and Techniques, P.B. 146, Mohammedia, Hassan II University of Casablanca, Morocco
María Dolores Ibáñez: Department of Pharmacology, Faculty of Pharmacy, University of Valencia, Avenue Vicent Andres Estelles s/n, 46100 Burjasot, Valencia, Spain
María Amparo Blázquez: Department of Pharmacology, Faculty of Pharmacy, University of Valencia, Avenue Vicent Andres Estelles s/n, 46100 Burjasot, Valencia, Spain
Mohamed Akssira: Laboratory of Physical Chimistry & Bioorganic Chemistry, URAC22, Faculty of Sciences and Techniques, P.B. 146, Mohammedia, Hassan II University of Casablanca, Morocco
Khalid Zerouali: Laboratory of Bacteriology, Virology and Hygiene, Ibn Rochd University Hospital, Casablanca, Morocco; Laboratory of Microbiology, Faculty of Medicine and Pharmacy, Hassan II University of Casablanca, Morocco
Naima Rhallabi: Research Unit of Microbiology and Bioactive Molecules, Laboratory of Virology, Microbiology, Quality, Biotechnology, Eco-toxicology and Biodiversity, Faculty of Sciences and Techniques, P.B. 146, Mohammedia, Hassan II University of Casablanca, Morocco

Journal volume & issue: Vol. 3
pp. 1 – 5

Abstract

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The aim of the present study was to determine the chemical composition, antibacterial and antibiofilm activities of the essential oil of sawdust from root burl wood of T. articulata. Nine strains including 8 clinical isolates from catheters-related infections and one ATCC 25923 were tested. Twenty-seven different compounds were identified by GC–MS in the essential oil of T. articulata extracted by hydrodistillation. Carvacrol and cedrol were the principal compounds representing 34.37 ± 0.07 and 11.07 ± 0.09 respectively. The EO had a strong antibacterial activity against all strains including one Multidrug-Resistant (MDR) and two Methicillin-Resistant S. aureus strains. Inhibition Zone Diameter (IZD) values ranged from 25 to 44 mm and the MIC values, determinated by microdilution method, ranged from 0.19 to 48 nL/mL. The EO had bactericidal effect and also exhibited at sub-lethal concentrations a very important inhibitory effect of biofilm formation of all strains.

Published in Current Research in Biotechnology

ISSN: 2590-2628 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://www.journals.elsevier.com/current-research-in-biotechnology

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