A chemo-enzymatic oxidation cascade to activate C–H bonds with in situ generated H2O2

Simon J. Freakley; Svenja Kochius; Jacqueline van Marwijk; Caryn Fenner; Richard J. Lewis; Kai Baldenius; Sarel S. Marais; Diederik J. Opperman; Susan T. L. Harrison; Miguel Alcalde; Martha S. Smit; Graham J. Hutchings

doi:10.1038/s41467-019-12120-w

Nature Communications (Sep 2019)

A chemo-enzymatic oxidation cascade to activate C–H bonds with in situ generated H2O2

Simon J. Freakley,
Svenja Kochius,
Jacqueline van Marwijk,
Caryn Fenner,
Richard J. Lewis,
Kai Baldenius,
Sarel S. Marais,
Diederik J. Opperman,
Susan T. L. Harrison,
Miguel Alcalde,
Martha S. Smit,
Graham J. Hutchings

Affiliations

Simon J. Freakley: Cardiff Catalysis Institute, School of Chemistry, Cardiff University, Main Building, Park Place
Svenja Kochius: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State
Jacqueline van Marwijk: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State
Caryn Fenner: South African DST-NRF Centre of Excellence in Catalysis, C*Change, University of Cape Town, Private Bag, Rondebosch
Richard J. Lewis: Cardiff Catalysis Institute, School of Chemistry, Cardiff University, Main Building, Park Place
Kai Baldenius: BASF SE, RBW/OS - A 30
Sarel S. Marais: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State
Diederik J. Opperman: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State
Susan T. L. Harrison: South African DST-NRF Centre of Excellence in Catalysis, C*Change, University of Cape Town, Private Bag, Rondebosch
Miguel Alcalde: Department of Biocatalysis, Institute of Catalysis, CSIC
Martha S. Smit: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State
Graham J. Hutchings: Cardiff Catalysis Institute, School of Chemistry, Cardiff University, Main Building, Park Place

DOI: https://doi.org/10.1038/s41467-019-12120-w
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 8

Abstract

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Continuous low-level supply or in situ generation of hydrogen peroxide (H2O2) is essential for the stability of unspecific peroxygenases. Here, the authors demonstrate that AuPd / TiO2 can generate sufficient H2O2 for the engineered unspecific heme-thiolate peroxygenase PaDa-I to oxidise a range of C-H bonds.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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