Biomolecular Concepts (Oct 2021)

Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells

  • Potter Matthew L.,
  • Smith Kathryn,
  • Vyavahare Sagar,
  • Kumar Sandeep,
  • Periyasamy-Thandavan Sudharsan,
  • Hamrick Mark,
  • Isales Carlos M.,
  • Hill William D.,
  • Fulzele Sadanand

DOI
https://doi.org/10.1515/bmc-2021-0015
Journal volume & issue
Vol. 12, no. 1
pp. 132 – 143

Abstract

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Stromal cell-derived factor 1 (SDF-1) is known to influence bone marrow stromal cell (BMSC) migration, osteogenic differentiation, and fracture healing. We hypothesize that SDF-1 mediates some of its effects on BMSCs through epigenetic regulation, specifically via microRNAs (miRNAs). MiRNAs are small non-coding RNAs that target specific mRNA and prevent their translation. We performed global miRNA analysis and determined several miRNAs were differentially expressed in response to SDF-1 treatment. Gene Expression Omnibus (GEO) dataset analysis showed that these miRNAs play an important role in osteogenic differentiation and fracture healing. KEGG and GO analysis indicated that SDF-1 dependent miRNAs changes affect multiple cellular pathways, including fatty acid biosynthesis, thyroid hormone signaling, and mucin-type O-glycan biosynthesis pathways. Furthermore, bioinformatics analysis showed several miRNAs target genes related to stem cell migration and differentiation. This study's findings indicated that SDF-1 induces some of its effects on BMSCs function through miRNA regulation.

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