Emersion-Associated Responses of an Intertidal Coral and Its Suitability for Transplantation to Ecologically Engineer Seawalls

Abstract

There is a growing interest in transplanting corals onto the intertidal section of artificial coastal defences (e.g., seawalls) as an ecological engineering strategy to enhance biodiversity on urban shores. However, this inevitably results in exposure to the harsh environmental conditions associated with emersion (aerial exposure). Although the effects of a multitude of environmental stressors on corals have been examined, their photophysiological and gene expression responses to emersion stress remain understudied, as does the among-genotype variation in these responses. In this study, we conducted an in situ experiment to test the effects of increased daily emersion duration on a locally common intertidal coral, Dipsastraea cf. lizardensis. Coral fragments (n = 3) from five genotypically distinct colonies were subjected to two treatments: (1) increased daily emersion duration (~4.5 h d−1) and, (2) control (~3 h d−1) for three consecutive days during spring low tide. We examined the post-experimental photophysiological responses and expression level of a stress-associated gene, Hsp16. Relative to the controls, coral fragments that were exposed to longer daily emersion duration displayed significantly reduced effective quantum yield, while endosymbiont density varied significantly among genotypes across the experimental conditions. We found no significant differences in chlorophyll a concentration and Hsp16 gene expression level, suggesting that changes in these processes may be gradual and the duration of treatment that the corals were subjected to is likely within their tolerance limits. Taken together, it appears that D. cf. lizardensis displays substantial capacity to cope with sup-optimal conditions associated with emersion which makes it a promising candidate for transplantation onto intertidal seawalls. However, within-species variation in their stress response indicates that not all genotypes respond similarly to emersion, and this should be taken into account when selecting donor colonies for transplantation.

Keywords

• air exposure
• coral gene expression
• photophysiology
• ecological engineering