Cellular macromolecules-tethered DNA walking indexing to explore nanoenvironments of chromatin modifications

Feng Chen; Min Bai; Xiaowen Cao; Jing Xue; Yue Zhao; Na Wu; Lei Wang; Dexin Zhang; Yongxi Zhao

doi:10.1038/s41467-021-22284-z

Nature Communications (Mar 2021)

Cellular macromolecules-tethered DNA walking indexing to explore nanoenvironments of chromatin modifications

Feng Chen,
Min Bai,
Xiaowen Cao,
Jing Xue,
Yue Zhao,
Na Wu,
Lei Wang,
Dexin Zhang,
Yongxi Zhao

Affiliations

Feng Chen: Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University
Min Bai: Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University
Xiaowen Cao: Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University
Jing Xue: Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University
Yue Zhao: Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University
Na Wu: Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University
Lei Wang: Department of Thoracic Surgery, Tangdu Hospital, Air Force Medical University
Dexin Zhang: Department of Respiratory Medicine, The Second Affiliated Hospital of Medical College, Xi’an Jiaotong University
Yongxi Zhao: Institute of Analytical Chemistry and Instrument for Life Science, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University

DOI: https://doi.org/10.1038/s41467-021-22284-z
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

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Investigation of spatial organization and relationships of biomolecules in cellular nanoenvironments is necessary to understand essential biological processes, but methodologically challenging. Here, the authors report cellular macromolecules-tethered DNA walking indexing (Cell-TALKING) to probe the nanoenvironments of DNA modifications around histone post-translational modifications, and explore the nanoenvironments in different cancer cell lines and clinical specimens.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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