Detection of attaching and effacing virulence gene of E. coli

Abstract

AbstrakLatar belakang: Bakteri Escherichia coli (E. coli) ada yang telah bermutasi menjadi patogen yang menimbulkan berbagai penyakit seperti hemorrhagic colitis (HC), hemolytic uremic syndrome (HUS), sepsis, pnemonia, neonatal meningitis, dan infeksi saluran kemih. Mutasi terjadi karena bakteri ini menerima transfer gen yang virulen dari bakteri lain yang hidup di sekitarnya. E. coli yang biasanya hidup normal di dalam usus manusia telah beradaptasi sehingga bisa hidup di tanah, makanan, dan saluran kemih. Penelitian ini mendeteksi gene yang virulen pada DNA isolat E. coli. Metode: Untuk deteksi E. coli yang virulen pada penelitian ini digunakan metode Real-time PCR dengan mencocokkan hasil sekuensing dengan sekuens E. coli virulen yang telah di publikasikan sebagai rujukan. Hasil: Sekuens RT PCR menggambarkan DNA gen eae pada BLAST mempunyai kesesuaian dengan rujukan segmen E. coli yang virulen. Dari sampel yang berasal dari E. coli di sekitar perairan lingkungan didapatkan gen Eae sebagai gen yang menyebabkan E. coli menjadi virulen sebesar 7,3%. Kesimpulan: E. coli yang virulen ditemukan pada sampel E. coli yang berasal dari perairan lingkungan dengan metode realtime PCR. (Health Science Indones 2013;1:41-6) Kata kunci: gen virulen E. coli, real-time PCR, perairan lingkunganAbstractBackground: Escherichia coli(E. coli) bacteria have developed into pathogenic bacteria that caused diseases such as hemorrhagic colitis (HC), hemolytic uremic syndrome (HUS), sepsis, pneumonia, neonatal meningitis, and urinary tract infections. Pathogenic E. coli have acquired pathogenic/virulence genes from other bacteria in their environment. E. coli that normally lived in the human gut had adapted to other niches such as soil, food and the urinary tract. This study investigated the presence of pathogenic E. coli from water samples by examining E. coli virulence genes present in E. coli genomes of water sourced isolates. Methods:This study used Real-time PCR to detect virulent E. coli and sequencing which was then matched to virulent E. coli listed in BLAST as a reference.Results: E. coli sequence from RT PCR was matched with eae DNA segment pattern in BLAST. E. coli isolates tested with eae gene were detected in 7.3% of E. coli strains isolated from environmental water sources. Conclusion:The presence of virulent E. coli strains was detected in environmental water sources using Real time PCR. (Health Science Indones 2013;1:41-6) Keywords: E. coli virulence genes, real-time PCR, environmental water

Keywords

• E. coli virulence genes, real-time PCR, environmental water