iScience (Aug 2023)
Integrated analysis of whole blood oxylipin and cytokine responses after bacterial, viral, and T cell stimulation reveals new immune networks
- Etienne Villain,
- Aurélie Chanson,
- Malwina Mainka,
- Nadja Kampschulte,
- Pauline Le Faouder,
- Justine Bertrand-Michel,
- Marion Brandolini-Bulon,
- Bruno Charbit,
- Munyaradzi Musvosvi,
- Nicole Bilek,
- Thomas J. Scriba,
- Lluis Quintana-Murci,
- Nils Helge Schebb,
- Darragh Duffy,
- Cécile Gladine,
- Laurent Abel,
- Andres Alcover,
- Hugues Aschard,
- Philippe Bousso,
- Nollaig Bourke,
- Petter Brodin,
- Pierre Bruhns,
- Nadine Cerf-Bensussan,
- Ana Cumano,
- Christophe D’Enfert,
- Ludovic Deriano,
- Marie-Agnès Dillies,
- James Di Santo,
- Gérard Eberl,
- Jost Enninga,
- Jacques Fellay,
- Ivo Gomperts-Boneca,
- Milena Hasan,
- Gunilla Karlsson Hedestam,
- Serge Hercberg,
- Molly A. Ingersoll,
- Olivier Lantz,
- Rose Anne Kenny,
- Mickaël Ménager,
- Hugo Mouquet,
- Cliona O'Farrelly,
- Etienne Patin,
- Sandra Pellegrini,
- Antonio Rausell,
- Frédéric Rieux-Laucat,
- Lars Rogge,
- Magnus Fontes,
- Anavaj Sakuntabhai,
- Olivier Schwartz,
- Benno Schwikowski,
- Spencer Shorte,
- Frédéric Tangy,
- Antoine Toubert,
- Mathilde Touvier,
- Marie-Noëlle Ungeheuer,
- Christophe Zimmer,
- Matthew L. Albert,
- Darragh Duffy,
- Lluis Quintana-Murci
Affiliations
- Etienne Villain
- Institut Pasteur, Université Paris Cité, Translational Immunology Unit, Paris, France
- Aurélie Chanson
- Université Clermont Auvergne, INRAE, UNH, Clermont-Ferrand, France
- Malwina Mainka
- Chair of Food Chemistry, Faculty of Mathematics and Natural Sciences, University of Wuppertal, Wuppertal, Germany
- Nadja Kampschulte
- Chair of Food Chemistry, Faculty of Mathematics and Natural Sciences, University of Wuppertal, Wuppertal, Germany
- Pauline Le Faouder
- MetaToul, MetaboHUB, Inserm/UPS UMR 1048-I2MC, Institut des Maladies Métaboliques et Cardiovasculaires, 31400 Toulouse, France
- Justine Bertrand-Michel
- MetaToul, MetaboHUB, Inserm/UPS UMR 1048-I2MC, Institut des Maladies Métaboliques et Cardiovasculaires, 31400 Toulouse, France
- Marion Brandolini-Bulon
- Université Clermont Auvergne, INRAE, UNH, Clermont-Ferrand, France; Université Clermont Auvergne, INRAE, UNH, Plateforme D’Exploration Du Métabolisme, MetaboHUB Clermont, Clermont-Ferrand, France
- Bruno Charbit
- Institut Pasteur, Université Paris Cité, CBUTechS, Paris, France
- Munyaradzi Musvosvi
- South African Tuberculosis Vaccine Initiative (SATVI), Division of Immunology, Department of Pathology and Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa
- Nicole Bilek
- South African Tuberculosis Vaccine Initiative (SATVI), Division of Immunology, Department of Pathology and Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa
- Thomas J. Scriba
- South African Tuberculosis Vaccine Initiative (SATVI), Division of Immunology, Department of Pathology and Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa
- Lluis Quintana-Murci
- Institut Pasteur, Université Paris Cité, CNRS UMR2000, Human Evolutionary Genetics Unit, Paris, France; Collège de France, 75005 Paris, France
- Nils Helge Schebb
- Chair of Food Chemistry, Faculty of Mathematics and Natural Sciences, University of Wuppertal, Wuppertal, Germany
- Darragh Duffy
- Institut Pasteur, Université Paris Cité, Translational Immunology Unit, Paris, France; Institut Pasteur, Université Paris Cité, CBUTechS, Paris, France
- Cécile Gladine
- Université Clermont Auvergne, INRAE, UNH, Clermont-Ferrand, France; Corresponding author
- Laurent Abel
- Andres Alcover
- Hugues Aschard
- Philippe Bousso
- Nollaig Bourke
- Petter Brodin
- Pierre Bruhns
- Nadine Cerf-Bensussan
- Ana Cumano
- Christophe D’Enfert
- Ludovic Deriano
- Marie-Agnès Dillies
- James Di Santo
- Gérard Eberl
- Jost Enninga
- Jacques Fellay
- Ivo Gomperts-Boneca
- Milena Hasan
- Gunilla Karlsson Hedestam
- Serge Hercberg
- Molly A. Ingersoll
- Olivier Lantz
- Rose Anne Kenny
- Mickaël Ménager
- Hugo Mouquet
- Cliona O'Farrelly
- Etienne Patin
- Sandra Pellegrini
- Antonio Rausell
- Frédéric Rieux-Laucat
- Lars Rogge
- Magnus Fontes
- Anavaj Sakuntabhai
- Olivier Schwartz
- Benno Schwikowski
- Spencer Shorte
- Frédéric Tangy
- Antoine Toubert
- Mathilde Touvier
- Marie-Noëlle Ungeheuer
- Christophe Zimmer
- Matthew L. Albert
- Darragh Duffy
- Lluis Quintana-Murci
- Journal volume & issue
-
Vol. 26,
no. 8
p. 107422
Abstract
Summary: Oxylipins are major immunomodulating mediators, yet studies of inflammation focus mainly on cytokines. Here, using a standardized whole-blood stimulation system, we characterized the oxylipin-driven inflammatory responses to various stimuli and their relationships with cytokine responses. We performed a pilot study in 25 healthy individuals using 6 different stimuli: 2 bacterial stimuli (LPS and live BCG), 2 viral stimuli (vaccine-grade poly I:C and live H1N1 attenuated influenza), an enterotoxin superantigen and a Null control. All stimuli induced a strong production of oxylipins but most importantly, bacterial, viral, and T cell immune responses show distinct oxylipin signatures. Integration of the oxylipin and cytokine responses for each condition revealed new immune networks improving our understanding of inflammation regulation. Finally, the oxylipin responses and oxylipin-cytokine networks were compared in patients with active tuberculosis or with latent infection. This revealed different responses to BCG but not LPS stimulation highlighting new regulatory pathways for further investigations.
