Do Media Extracellular Vesicles and Extracellular Vesicles Bound to the Extracellular Matrix Represent Distinct Types of Vesicles?
Saida Mebarek,
Rene Buchet,
Slawomir Pikula,
Agnieszka Strzelecka-Kiliszek,
Leyre Brizuela,
Giada Corti,
Federica Collacchi,
Genevieve Anghieri,
Andrea Magrini,
Pietro Ciancaglini,
Jose Luis Millan,
Owen Davies,
Massimo Bottini
Affiliations
Saida Mebarek
Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, UMR CNRS 5246, Université de Lyon, Université Claude Bernard Lyon 1, 69 622 Villeurbanne Cedex, France
Rene Buchet
Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, UMR CNRS 5246, Université de Lyon, Université Claude Bernard Lyon 1, 69 622 Villeurbanne Cedex, France
Slawomir Pikula
Laboratory of Biochemistry of Lipids, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, Poland
Agnieszka Strzelecka-Kiliszek
Laboratory of Biochemistry of Lipids, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warsaw, Poland
Leyre Brizuela
Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, UMR CNRS 5246, Université de Lyon, Université Claude Bernard Lyon 1, 69 622 Villeurbanne Cedex, France
Giada Corti
Department of Experimental Medicine, University of Rome Tor Vergata, 00133 Rome, Italy
Federica Collacchi
Department of Experimental Medicine, University of Rome Tor Vergata, 00133 Rome, Italy
Genevieve Anghieri
School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough LE113TU, UK
Andrea Magrini
Department of Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy
Pietro Ciancaglini
Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14040-901, São Paulo, Brazil
Jose Luis Millan
Sanford Children’s Health Research Center, Sanford Burnham Prebys, La Jolla, CA 92037, USA
Owen Davies
School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough LE113TU, UK
Massimo Bottini
Department of Experimental Medicine, University of Rome Tor Vergata, 00133 Rome, Italy
Mineralization-competent cells, including hypertrophic chondrocytes, mature osteoblasts, and osteogenic-differentiated smooth muscle cells secrete media extracellular vesicles (media vesicles) and extracellular vesicles bound to the extracellular matrix (matrix vesicles). Media vesicles are purified directly from the extracellular medium. On the other hand, matrix vesicles are purified after discarding the extracellular medium and subjecting the cells embedded in the extracellular matrix or bone or cartilage tissues to an enzymatic treatment. Several pieces of experimental evidence indicated that matrix vesicles and media vesicles isolated from the same types of mineralizing cells have distinct lipid and protein composition as well as functions. These findings support the view that matrix vesicles and media vesicles released by mineralizing cells have different functions in mineralized tissues due to their location, which is anchored to the extracellular matrix versus free-floating.
