Conformational motions and ligand-binding underlying gating and regulation in IP3R channel

Guizhen Fan; Mariah R. Baker; Lara E. Terry; Vikas Arige; Muyuan Chen; Alexander B. Seryshev; Matthew L. Baker; Steven J. Ludtke; David I. Yule; Irina I. Serysheva

doi:10.1038/s41467-022-34574-1

Nature Communications (Nov 2022)

Conformational motions and ligand-binding underlying gating and regulation in IP3R channel

Guizhen Fan,
Mariah R. Baker,
Lara E. Terry,
Vikas Arige,
Muyuan Chen,
Alexander B. Seryshev,
Matthew L. Baker,
Steven J. Ludtke,
David I. Yule,
Irina I. Serysheva

Affiliations

Guizhen Fan: Department of Biochemistry and Molecular Biology, Structural Biology Imaging Center, McGovern Medical School at The University of Texas Health Science Center at Houston
Mariah R. Baker: Department of Biochemistry and Molecular Biology, Structural Biology Imaging Center, McGovern Medical School at The University of Texas Health Science Center at Houston
Lara E. Terry: Department of Pharmacology and Physiology, School of Medicine and Dentistry, University of Rochester
Vikas Arige: Department of Pharmacology and Physiology, School of Medicine and Dentistry, University of Rochester
Muyuan Chen: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine
Alexander B. Seryshev: Department of Biochemistry and Molecular Biology, Structural Biology Imaging Center, McGovern Medical School at The University of Texas Health Science Center at Houston
Matthew L. Baker: Department of Biochemistry and Molecular Biology, Structural Biology Imaging Center, McGovern Medical School at The University of Texas Health Science Center at Houston
Steven J. Ludtke: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine
David I. Yule: Department of Pharmacology and Physiology, School of Medicine and Dentistry, University of Rochester
Irina I. Serysheva: Department of Biochemistry and Molecular Biology, Structural Biology Imaging Center, McGovern Medical School at The University of Texas Health Science Center at Houston

DOI: https://doi.org/10.1038/s41467-022-34574-1
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 15

Abstract

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Here authors report cryo-EM structures of IP3R1 which provide atomic details of IP3, Ca2+ and ATP binding. Molecular motions of key domains and sidechains were found to regulate ligand binding and gating, which are validated by functional assays.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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