Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2

Jiuyang Liu; Zhaoyu Xue; Kendra R. Vann; Xiaobing Shi; Tatiana G. Kutateladze

STAR Protocols (Dec 2020)

Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2

Jiuyang Liu,
Zhaoyu Xue,
Kendra R. Vann,
Xiaobing Shi,
Tatiana G. Kutateladze

Affiliations

Jiuyang Liu: Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA
Zhaoyu Xue: Center for Epigenetics, Van Andel Research Institute, Grand Rapids, MI 49503, USA
Kendra R. Vann: Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA
Xiaobing Shi: Center for Epigenetics, Van Andel Research Institute, Grand Rapids, MI 49503, USA; Corresponding author
Tatiana G. Kutateladze: Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA; Corresponding author

Journal volume & issue: Vol. 1, no. 3
p. 100155

Abstract

Read online

Summary: Since its discovery, several ligands of the ZZ domain have been identified; however, molecular and structural information underlying binding of these ligands remains limited. Here, we describe a protocol for biochemical and structural analysis of the ZZ domain of human E3 ubiquitin ligase HERC2 (HERC2ZZ) and its interaction with its ligands: the N-terminal tails of histone H3 and SUMO1. This methodology could be applied for characterization of binding activities of other histone readers.For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

About the journal