The Expression of Stem Cell Marker LGR5 and Its Coexpression with Β-Catenin in Sporadic Colorectal Carcinoma and Adenoma: A Comparative Immunohistochemical Study
Eman Mohamed Ahmed,
Abeer Said Farag,
Mohammed S. Abdelwahed,
Mehenaz Hanbazazh,
Abdulhadi Samman,
Diaa Ashmawy,
Nageh Rady Abd-Elhameed,
Mohamed Tharwat,
Alyaa E. Othman,
Taiseer Ahmed Shawky,
Radwa Mohamed Attia,
Adel Abdelwahid Ibrahim,
Sherif Azzam,
Mohammed E. A. Elhussiny,
Mohamed Nasr,
Suhaib Alsayed Naeem,
Wagih M. Abd-Elhay,
Ahmed Mohammad Ali Alfaifi,
Abdulkarim Hasan
Affiliations
Eman Mohamed Ahmed
Pathology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo 11884, Egypt
Abeer Said Farag
Pathology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo 11884, Egypt
Mohammed S. Abdelwahed
Pathology Department, Faculty of Medicine, Al-Azhar University, Cairo 11884, Egypt
Mehenaz Hanbazazh
Pathology Department, Faculty of Medicine, University of Jeddah, Jeddah 23218, Saudi Arabia
Abdulhadi Samman
Pathology Department, Faculty of Medicine, University of Jeddah, Jeddah 23218, Saudi Arabia
Diaa Ashmawy
Pathology Department, Faculty of Medicine, Al-Azhar University, Damietta 34517, Egypt
Nageh Rady Abd-Elhameed
Pathology Department, Faculty of Medicine, Al-Azhar University, Assiut 71524, Egypt
Mohamed Tharwat
Pathology Department, Faculty of Medicine, Al-Azhar University, Assiut 71524, Egypt
Background: LGR5 is one of the most important stem cell markers for colorectal cancer (CRC), as it potentiates Wnt/Β-catenin signaling. The well-characterized deregulation of Wnt/Β-catenin signaling that occurs during adenoma/carcinoma sequence in CRC renders LGR5 a hopeful therapeutic target. We assessed the immunohistochemical expression of LGR5 and Β-catenin in normal colonic and tumorous lesions with a clinicopathological correlation. Methods: Tissue blocks and clinical data of 50 selected cases were included: 8 from normal mucosa, 12 cases of adenoma, and 30 cases of CRC, where sections were cut and re-examined and the immunohistochemical technique was conducted using anti-LGR5 and anti-Β-catenin to measure the staining density. Results: There was no expression of LGR5 in normal mucosa compared to samples of adenoma and CRC samples. The association analysis showed that CRC specimens were more likely to have strong LGR5 and Β-catenin expressions than the other two groups (p = 0.048 and p p = 0.013 and p = 0.036, respectively). In contrast, there were no statistically significant associations between LGR5 and Β-catenin expression with grade and stage. Conclusion: These results suggest and support the possible role of LGR5 as a potential marker of cancer stem cells in sporadic colorectal carcinogenesis in addition to a prognostic value for LGR5 and Β-catenin in adenomatous lesions according to immunohistochemical expression density. A potential therapeutic role of LGR5 in CRC is suggested for future studies based on its role in pathogenesis.
