EFFECT OF LACTOBACILLI EXOPOLYSACCHARIDES ON PHAGOCYTE AND CYTOKINE ACTIVITY IN VITRO AND IN ANIMAL ORGANISM DURING INFECTIOUS PROCESS MODELING

Abstract

Aim. Study the effect of lactobacilli exopolysaccharides (EPS) on cytokine and phagocyte activity in vitro and in mice organism during modelling of an infectious process. Materials and methods. Lactobacillus delbrueckiisubsp. delbrueckii B-1596 (laksaran 1596), L. delbrueckii B-1936 (laksaran 1936) and L. delbrueckii ssp. bulgaricus (laksaran Z) were used in the study. EPS were administered into white mice 1 hour after the Staphylococcus aureus 209-P infection. Index of phagocyte completion and index of killing activation (IKA) were calculated during phagocyte activity study. IL-1a, TNF-α, IFN-γ and IL-4 cytokine content was determined in blood sera and macrophage supernatants. Results. Laksaran 1596, 1936 and Z had ambiguous effect on cytokine production. Laksaran Z and 1936, 6 hours after mice infection increased IL-1 content in blood sera. Laksaran Z had the most pronounced effect on macrophages, resulting in an increase of active macrophages, facilitating increased digestion of S. aureus 209-Р and IKA increase, stimulated cytokine production. Conclusion. The results obtained allow to speak about a possibility of using laksaran Z as a prophylaxis immune modulating preparation for correction of animal cytokine status.

Keywords

• exopolysaccharides
• lactobacilli
• laksarans
• phagocyte and cytokine activity