Drug Design, Development and Therapy (Feb 2020)
Inhibitory Effect of Curcumin on Artery Restenosis Following Carotid Endarterectomy and Its Associated Mechanism in vitro and in vivo
Abstract
Dapeng Zhang,1 Yanhui Yang,2 Yuanchao Li,1 Guodong Zhang,1 Zhenguo Cheng1 1Neurosurgery Department, Xinxiang Central Hospital, Xinxiang, Henan Province 453000, People’s Republic of China; 2Color Ultrasonic Room, People’s Hospital of Huixian, Xinxiang, Henan Province 453600, People’s Republic of ChinaCorrespondence: Zhenguo ChengNeurosurgery Department, Xinxiang Central Hospital, No. 56 Jinsui Street, Xinxiang, Henan Province 453000, People’s Republic of ChinaTel +86 156 3735 9907Email [email protected]: The present study aimed to assess the effect of curcumin (Cur) on carotid artery restenosis following carotid endarterectomy (CEA) and its associated mechanism in vivo and in vitro.Methods: Ang II was used to induce excessive proliferation of rabbit aortic smooth muscle cells (CCC-SMC-1) in order to establish a hemadostenosis cell model. Similarly, the animal model of carotid artery restenosis was established by carotid artery gas drying injury combined with high-fat feed prior to CEA. CCC-SMC-1 cells and animals were treated by Cur and its effects on neointimal hyperplasia, inflammation and oxidative stress were detected and observed. The proteins that were associated with the Raf/MEK/ERK pathway were detected in cells and rabbit carotid artery tissues.Results: Cur inhibited the proliferation of smooth muscle cells and neointimal formation and reduced the inflammation and oxidative stress indices. Concomitantly, Cur reduced the phosphorylation of the Raf/MEK/ERK pathway proteins.Conclusion: Cur could inhibit carotid restenosis following CEA by inhibiting the activation of the Raf/MEK/ERK pathway.Keywords: curcumin, carotid endarterectomy, restenosis, vascular smooth muscle cells, Raf/MEK/ERK
