Multiplex primers employment for detection of rinderpest virus RNA by PCR

Abstract

Given the high contagiousness and rapid spread of the rinderpest virus, timely and accurate diagnosis plays a key role in preventing epidemics and taking measures to control the disease. The study aims to evaluate the efficiency of using multiplex primers in the polymerase chain reaction method for the detection of rinderpest virus ribonucleic acid. The study included the analysis of samples such as blood serum and conjunctival swabs from 50 animals with clinical manifestations of the disease. The experiment involved the collection of clinical samples such as blood serum and conjunctival washings. The results demonstrate the high specificity of the developed primers. These primers stand out because they use two pairs of the same gene region with different variable sequences that are specific for all strains of the rinderpest virus. In the polymerase chain reaction, both pairs of primers are used simultaneously at equal concentrations and under the same conditions. An additional polymerase chain reaction performed using these primers at the optimal annealing temperature confirmed the successful amplification and specificity of the primers. The absence of dimers and nonspecific products in the negative control confirmed the purity and reliability of the results. Thus, these results demonstrate that the use of these multiplex polymerase chain reaction primers allows for the efficient detection of the ribonucleic acid of the rinderpest virus of different strains. The developed multiplex primers represent an innovative method for the diagnosis of rinderpest virus with the potential for use in veterinary practice and animal disease control

Keywords

• oligonucleotides
• specificity
• annealing
• diagnostic efficiency
• biological samples
• amplification