Deciphering genetic diversity phylogeny and assembly of Allium species through micro satellite markers on nuclear DNA
Talamarla Yeswanth Mahidar Gowd,
Chandra Deo,
Dalasanuru Chandregowda Manjunathagowda,
Vijay Mahajan,
Ram Dutta,
Nangsol Dolma Bhutia,
Barun Singh,
Vadde Mounika
Affiliations
Talamarla Yeswanth Mahidar Gowd
Department of Vegetable Science, College of Horticulture and Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh, India; ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune, Maharashtra, India
Chandra Deo
Department of Vegetable Science, College of Horticulture and Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh, India
Dalasanuru Chandregowda Manjunathagowda
ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune, Maharashtra, India; Division of Vegetable Crops, ICAR-Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru, Karnataka, India; Corresponding author. ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune, Maharashtra, India.
Vijay Mahajan
ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune, Maharashtra, India; Corresponding author.
Ram Dutta
ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune, Maharashtra, India
Nangsol Dolma Bhutia
Department of Vegetable Science, College of Horticulture and Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh, India
Barun Singh
Department of Fruit Science, College of Horticulture and Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh, India
Vadde Mounika
Department of Vegetable Science, College of Horticulture and Forestry, Central Agricultural University, Pasighat, Arunachal Pradesh, India
The genus Allium is the most diverse, with cultivated crops such as onion, garlic, bunching onion, chives, leeks, and shallots, and several wild and semi-domesticated Allium species utilized as minor vegetables. These minor species are the genetic resources for various abiotic and biotic stresses. To employ underutilized species in breeding programmes, the magnitude of the genetic background of cultivated and semi-domesticated alliums, the phylogeny and diversity of the population must be known. In this study, nineteen SSR markers were employed to study the divergence and population structure of 95 Allium accessions which includes species, varieties, and interspecific hybrids, yielded 92 polymorphic loci, averaging 4.84 loci per SSR. PIC values range between 0.24 (ACM 018) and 0.98 (ACM 099). The cross transferability of ACM markers among Allium species ranges from 1.33 to 10.53 per cent, which is relatively low. The genotypes investigated were clustered into four primary clusters A, B, C, and D with 13 sub clusters I-XIII, conferring to the clustering results. The population structure investigations also found that K is a peak at value 4, implying that the population is predominantly segregated into four distinct groups, which associates the clustering pattern. The employed SSR markers adeptly unravel the complexities of diversity within alliums, holding promise for refining future breeding programs targeting elite progenies.
