Micrometam C Protects against Oxidative Stress in Inflammation Models in Zebrafish and RAW264.7 Macrophages
Hao Tang,
Hui Ge,
Zhi-Bin Chen,
Xiong-Ming Luo,
Feng-Juan Su,
Yan-Bing Liang,
Zhen-Yu Li,
Jing-Guo Wu,
Qing Yang,
Li-Jin Zeng,
Zhong-Fu Ma
Affiliations
Hao Tang
Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
Hui Ge
Department of Health Care Clinic, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
Zhi-Bin Chen
Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
Xiong-Ming Luo
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
Feng-Juan Su
Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
Yan-Bing Liang
Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
Zhen-Yu Li
Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
Jing-Guo Wu
Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
Qing Yang
Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
Li-Jin Zeng
Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
Zhong-Fu Ma
Department of General Internal Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
Micrometam C is a core of novel marine compound isolated from the mangrove associates Micromelum falcatum. In this study, we investigated the protective effects of micrometam C in inflammation models in the transgenic zebrafish line Tg (corola: eGFP) and RAW264.7 macrophages. We found that micrometam C significantly suppressed the migration of immune cells in tail-cutting-induced inflammation in transgenic zebrafish and reduced lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) in both zebrafish and macrophages. In addition, micrometam C also restored LPS-induced reduction of endogenous antioxidants, such as catalase (CAT), glutathione (GSH) and superoxide dismutase (SOD). The protective effects of micrometam C were in parallel to its inhibition of NADPH oxidase and nuclear factor-kappa-binding (NF-κB) activity. Thus, the present results demonstrate that micrometam C protects against LPS-induced inflammation possibly through its antioxidant property.
