Application of colloidal gold immunochromatography on rapid detection of quinolones in bean sprouts

Abstract

ObjectiveTo study the applicability for determination of norfloxacin， ofloxacin， difloxacin， enrofloxacin， ciprofloxacin， fleroxacin， dafloxacin， pefloxacin， lomefloxacin and salafloxacin in bean sprouts by colloidal gold immunochromatography.MethodsTook representative bean sprout ground 2 g， extracted with 5 mL extraction solution， and then filtered and diluted 2 times. Drew the diluent into the gold standard micropore， incubated at room temperature， and inserted the test strip into the gold standard micropore. The reaction results were interpreted by comparing the color depth of the detection line （T line） and the control line （C line）. The experiment also studied the cross-reaction of 6-benzyladenine， 4-chlorophenoxyacetic acid， metronidazole， urea and clopidourea， and the reliability of the method was investigated by real positive samples and multi-level spiked samples.ResultsThe detection limit of the method could reach 5 μg/kg ； no cross reactions occurred with 6-benzyladenine， 4-chlorophenoxyacetic acid， metronidazole， urea and clopidourea under the condition of routine detection level； The results of real positive samples and multi-level spiked samples confirmed that the method was reliable.ConclusionThe method is sensitive， accurate and fast， which is suitable for the field rapid detection of quinolones in bean sprouts.

Keywords

• colloidal gold immunochromatography
• quinolones
• bean sprouts
• rapid detection