Identification of biomarkers related to tryptophan metabolism in osteoarthritis

Junjun Yang; Ping Zhou; Tangbing Xu; Kaida Bo; Chenxin Zhu; Xu Wang; Jun Chang

Biochemistry and Biophysics Reports (Sep 2024)

Identification of biomarkers related to tryptophan metabolism in osteoarthritis

Junjun Yang,
Ping Zhou,
Tangbing Xu,
Kaida Bo,
Chenxin Zhu,
Xu Wang,
Jun Chang

Affiliations

Junjun Yang: Department of Orthopaedics, The First Affiliated Hospital of Anhui Medical University, Hefei, China; Department of Orthopaedics, Anhui Public Health Clinical Center, Hefei, China; Corresponding author. Department of Orthopaedics, The First Affiliated Hospital of Anhui Medical University; Anhui Public Health Clinical Center, Hefei, 230000, China.
Ping Zhou: Department of Orthopaedics, The First Affiliated Hospital of Anhui Medical University, Hefei, China; Department of Orthopaedics, Anhui Public Health Clinical Center, Hefei, China
Tangbing Xu: Department of Orthopaedics, The First Affiliated Hospital of Anhui Medical University, Hefei, China; Department of Orthopaedics, Anhui Public Health Clinical Center, Hefei, China
Kaida Bo: Department of Orthopaedics, The First Affiliated Hospital of Anhui Medical University, Hefei, China; Department of Orthopaedics, Anhui Public Health Clinical Center, Hefei, China
Chenxin Zhu: Department of Orthopaedics, The First Affiliated Hospital of Anhui Medical University, Hefei, China; Department of Orthopaedics, Anhui Public Health Clinical Center, Hefei, China
Xu Wang: Department of Orthopaedics, The First Affiliated Hospital of Anhui Medical University, Hefei, China; Department of Orthopaedics, Anhui Public Health Clinical Center, Hefei, China
Jun Chang: Department of Orthopaedics, The First Affiliated Hospital of Anhui Medical University, Hefei, China; Department of Orthopaedics, Anhui Public Health Clinical Center, Hefei, China; Corresponding author. Department of Orthopaedics, The First Affiliated Hospital of Anhui Medical University; Anhui Public Health Clinical Center, Hefei, 230000, China.

Journal volume & issue: Vol. 39
p. 101763

Abstract

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Background: OA (osteoarthritis) is a common joint disease characterized by damage to the articular cartilage and affects the entire joint tissue, with its main manifestations being joint pain, stiffness, and limited movement.Currently,we know that OA is a complex process composed of inflammatory and metabolic factors.It is reported that the occurrence and development of OA is related to the change of tryptophan metabolism.Therefore, the study of tryptophan metabolism and OA related genes is hopeful to find a new therapeutic target for OA. Methods: Differentially expressed genes (DEGs) in GSE55235 were gained via differential expression analysis (OA samples vs normal samples). The tryptophan metabolic related DEGs (TMR-DEGs) were obtained by overlapping tryptophan metabolism related genes (TMRGs) and DEGs. Further, biomarkers were screening via Least absolute shrinkage and selection operator (LASSO), naive bayes (NB) and supportvector machine-recursive feature elimination (SVM-RFE) algorithm to establish a diagnostic model. Afterward, Gene Set Enrichment Analysis (GSEA) and drug prediction were performed based on diagnostic biomarkers by multiple software and databases. Eventually, expression level of biomarker public databases was verified using real-time quantitative polymerase chain reaction (RT-qPCR). Results: Three tryptophan metabolism related biomarkers (TDO2, AOX1 and SLC3A2) were identified in OA. GSEA analysis demonstrated that biomarkers were associated with the function of ‘FoxO signaling pathway’, ‘spliceosome’ and ‘ribosome’. There were seven drugs with therapeutic potential on TDO2 and AOX1. Ultimately, compared with normal group, expression of AOX1 and SLC3A2 in OA group remarkable lower. Conclusion: Overall, three tryptophan metabolic related diagnostic biomarkers that associated with OA were obtained, which provided a original direction for the diagnosis and treatment of OA.

Published in Biochemistry and Biophysics Reports

ISSN: 2405-5808 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General); Science: Chemistry: Organic chemistry: Biochemistry
Website: http://www.journals.elsevier.com/biochemistry-and-biophysics-reports/

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