The impact of different coverslipping methods in the quality of the whole slide images used for diagnosis in pathology

Abstract

Digital pathology workflow aims to create whole slide images (WSIs) for diagnosis. The quality of the WSIs depends primarily on the quality of the glass slides produced by the pathology laboratory, where the coverslipping method plays an important role. In this study we compare the glass, the film, and the liquid coverslipping methods to evaluate which ones are suitable to create WSIs for diagnosis. The study included 18 formalin-fixed paraffin-embedded tissue blocks. Of each block, 3 consecutive sections were covered using 1 of the 3 methods. The slides were scanned and evaluated for quality criteria by 2 pathologists experienced in digital pathology. The coverslipping method interferes with the quality of the WSIs, as well as with the scanning time and the file size of the WSIs. All coverslipping methods were found suitable for diagnosis. The glass and liquid methods were manual and had similar results concerning the presence of air bubbles/polymer accumulation, air drying artefacts, tissue exposed, and staining alterations. The glass method was the one with more air bubbles. The liquid method was associated with more alterations on the WSIs, but with the lowest file sizes. Automation of coverslipping and calibration of the scanner for the coverslipping method chosen by the pathology laboratory are relevant for the final quality of the WSIs.

Keywords

• Coverslipping methods
• Film coverslip
• Glass coverslip
• Liquid coverslip
• Digital pathology
• Whole slide image