Construction of Bioreactors for Obtaining Mycelium from Agaricus Brunnescens and Pleurotus Ostreatus Mushrooms Using the Honey Tek Technique

Abstract

Abstract Mycelium cultivation plays an important role in mushroom production and in various biotechnological studies. Submerged fermentation has received much attention as a promising alternative for the efficient production of mushroom biomass and active metabolites. For the following research, bioreactors were constructed to obtain pure mycelium from Pleurotus ostreatus and Agaricus brunnescens mushrooms using the Honey technique. This method is based on using honey as the main nutrient for the growth of microorganisms on an industrial scale. In the beginning, spore impressions were extracted by the dry chamber method and inoculated into the honey broth. The bioreactors were dimensioned with a 2.5 L culture chamber, 2 L production, controlled temperature of 28○C, pH = 4, and agitation of 120 revolutions per minute. Conidia/milliliter was quantified every three days with a Neubauer chamber, and the Kjeldahl method was used to identify protein as the main bioactive metabolite of the mycelium. Finally, the mycelium obtained was evaluated on three types of substrates comparing their colonization time. The use of bioreactors in mycelium cultivation is efficient as better mycelium quality, higher biomass yield, and more dispersion on substrates are obtained. It also has the benefits of much better oxygen mass transfer and culture homogeneity. With this technique, physical, chemical, and biological factors can be controlled to produce mushrooms in a much shorter time.

Keywords

• bioreactor, honey technic, mycelial biomass, agaricus brunnescens, pleurotus ostreatus.